PMID- 28465620
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 May 2
TI  - IVIG regulates the survival of human but not mouse neutrophils.
PG  - 1296
LID - 10.1038/s41598-017-01404-0 [doi]
LID - 1296
AB  - Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the 
      pooled plasma from thousands of healthy donors and are being tested in 
      preclinical mouse models. Inherent challenges, however, are the pluripotency of 
      IVIG and its xenogeneicity in animals. IVIG can alter the viability of human 
      neutrophils via agonistic antibodies to Fas and Siglec-9. In this study, we 
      compared the effects of IVIG on human and mouse neutrophils using different death 
      assays. Different commercial IVIG preparations similarly induced 
      cytokine-dependent death in human neutrophils, whereas they had no effects on the 
      survival of either peripheral blood or bone marrow neutrophils from C57BL/6 or 
      BALB/c mice. F(ab')(2) but not Fc fragments of IVIG induced death of human 
      neutrophils, whereas neither of these IVIG fragments, nor agonistic monoclonal 
      antibodies to human Fas or Siglec-9 affected the viability of mouse neutrophils. 
      Pooled mouse IgG, which exhibited a different immunoprofile compared to IVIG, 
      also had no effect on mouse cells. Together, these observations demonstrate that 
      effects of IVIG on neutrophil survival are not adequately reflected in current 
      mouse models, despite the key role of these cells in human inflammatory and 
      autoimmune diseases.
FAU - Schneider, Christoph
AU  - Schneider C
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - Wicki, Simone
AU  - Wicki S
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - Graeter, Stefanie
AU  - Graeter S
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - Timcheva, Tankica M
AU  - Timcheva TM
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - Keller, Christian W
AU  - Keller CW
AD  - Institute of Experimental Immunology, Laboratory of Neuroinflammation, University 
      of Zurich, Zurich, Switzerland.
FAU - Quast, Isaak
AU  - Quast I
AUID- ORCID: 0000-0001-6517-8537
AD  - Institute of Experimental Immunology, Laboratory of Neuroinflammation, University 
      of Zurich, Zurich, Switzerland.
AD  - Department of Immunology and Pathology, Central Clinical School, Monash 
      University, Melbourne, Australia.
FAU - Leontyev, Danila
AU  - Leontyev D
AD  - Department of Medicine, University of Toronto and Centre for Innovation, Canadian 
      Blood Services, Toronto, Ontario, Canada.
FAU - Djoumerska-Alexieva, Iglika K
AU  - Djoumerska-Alexieva IK
AD  - Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian 
      Academy of Sciences, Sofia, Bulgaria.
FAU - Kasermann, Fabian
AU  - Kasermann F
AD  - CSL Behring, Research, Bern, Switzerland.
FAU - Jakob, Stephan M
AU  - Jakob SM
AD  - Department of Intensive Care Medicine, University Hospital Bern (Inselspital), 
      University of Bern, Bern, Switzerland.
FAU - Dimitrova, Petya A
AU  - Dimitrova PA
AD  - Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian 
      Academy of Sciences, Sofia, Bulgaria.
FAU - Branch, Donald R
AU  - Branch DR
AD  - Department of Medicine, University of Toronto and Centre for Innovation, Canadian 
      Blood Services, Toronto, Ontario, Canada.
FAU - Cummings, Richard D
AU  - Cummings RD
AD  - Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical 
      School, Boston, MA, USA.
FAU - Lunemann, Jan D
AU  - Lunemann JD
AD  - Institute of Experimental Immunology, Laboratory of Neuroinflammation, University 
      of Zurich, Zurich, Switzerland.
FAU - Kaufmann, Thomas
AU  - Kaufmann T
AUID- ORCID: 0000-0001-9906-874X
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - Simon, Hans-Uwe
AU  - Simon HU
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland.
FAU - von Gunten, Stephan
AU  - von Gunten S
AUID- ORCID: 0000-0002-2753-8738
AD  - Institute of Pharmacology, University of Bern, Bern, Switzerland. 
      stephan.vongunten@pki.unibe.ch.
LA  - eng
GR  - P41 GM103694/GM/NIGMS NIH HHS/United States
GR  - R24 GM098791/GM/NIGMS NIH HHS/United States
GR  - U54 GM062116/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170502
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (SIGLEC9 protein, human)
RN  - 0 (Sialic Acid Binding Immunoglobulin-like Lectins)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/administration & dosage/immunology
MH  - Antigens, CD/immunology
MH  - Cell Survival/*drug effects/immunology
MH  - Humans
MH  - Immunoglobulin Fc Fragments/immunology
MH  - Immunoglobulin G/administration & dosage/*immunology
MH  - Immunoglobulins, Intravenous/*administration & dosage/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neutrophils/*drug effects/immunology
MH  - Sialic Acid Binding Immunoglobulin-like Lectins/immunology
MH  - fas Receptor/immunology
PMC - PMC5430961
COIS- The authors declare that they have no competing interests.
EDAT- 2017/05/04 06:00
MHDA- 2018/09/11 06:00
PMCR- 2017/05/02
CRDT- 2017/05/04 06:00
PHST- 2016/06/01 00:00 [received]
PHST- 2017/03/30 00:00 [accepted]
PHST- 2017/05/04 06:00 [entrez]
PHST- 2017/05/04 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2017/05/02 00:00 [pmc-release]
AID - 10.1038/s41598-017-01404-0 [pii]
AID - 1404 [pii]
AID - 10.1038/s41598-017-01404-0 [doi]
PST - epublish
SO  - Sci Rep. 2017 May 2;7(1):1296. doi: 10.1038/s41598-017-01404-0.