PMID- 28466565
OWN - NLM
STAT- MEDLINE
DCOM- 20190625
LR  - 20190625
IS  - 1440-1681 (Electronic)
IS  - 0305-1870 (Linking)
VI  - 44 Suppl 1
DP  - 2017 Dec
TI  - Aging leads to impaired epicutaneous sensitization that causes attenuated allergy 
      and pulmonary inflammation in mice.
PG  - 64-69
LID - 10.1111/1440-1681.12778 [doi]
AB  - Aging is associated with altered decreased barrier function in the skin, which 
      can lead to different types of immunoglobulin E (IgE)-mediated sensitization to 
      environmental allergens. Yet, allergen-specific respiratory sensitization among 
      the elderly is not well described. The aim of this study was to investigate the 
      effect of aging on allergic pulmonary inflammation induced by epicutaneous 
      sensitization of mechanically irritated skin in mice. For this purpose, 6-week-, 
      6-month-, and 18-month-old female BALB/c mice, underwent epicutaneous 
      sensitization with ovalbumin (OVA) or phosphate buffered saline (PBS), followed 
      by an inhaled OVA challenge. Blood OVA-specific IgE levels measured after 
      epicutaneous sensitization, as well as, bronchial alveolar lavage fluids (BALF) 
      leucocyte, eosinophil, and cytokine levels measured after OVA inhalation 
      challenge were similar among the 6-week-old (young) and 6-month-old (adult) 
      groups. However, significantly decreased levels of systemic OVA IgE, and BALF 
      leukocyte, eosinophil and T helper cell type 2 cytokine levels, were measured 
      after OVA inhalation challenge in elderly (18-month-old) mice compared to the 
      other groups of mice. In addition, interleukin-10 (IL-10), a regulatory 
      suppressor cytokine, was more abundant in the BALF of the elderly group after 
      epicutaneous sensitization and inhalation challenge. Our results suggest that 
      elderly mice have a reduced allergic response to induced sensitization with OVA, 
      possibly regulated by increased IL-10 levels.
CI  - (c) 2017 John Wiley & Sons Australia, Ltd.
FAU - Langier, Sheila
AU  - Langier S
AUID- ORCID: 0000-0002-7338-9665
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Benor, Shira
AU  - Benor S
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Kadar, Laliv
AU  - Kadar L
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Shani, Nir
AU  - Shani N
AD  - Microsurgery and Plastic Surgery Laboratory of the Plastic Surgery Department, 
      Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Etkin, Sara
AU  - Etkin S
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Stark, Moshe
AU  - Stark M
AD  - Biochemistry Laboratory, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Bondar, Ekaterina
AU  - Bondar E
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Aizic, Asaf
AU  - Aizic A
AD  - Department of Pathology, Tel Aviv Medical Center, Tel Aviv, Israel.
FAU - Kivity, Shmuel
AU  - Kivity S
AD  - Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20171019
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 0 (IL10 protein, mouse)
RN  - 130068-27-8 (Interleukin-10)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Cutaneous
MH  - Age Factors
MH  - Aging/*immunology
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Disease Models, Animal
MH  - Eosinophils/immunology/metabolism
MH  - Female
MH  - Immunoglobulin E/blood/immunology
MH  - Interleukin-10/immunology/metabolism
MH  - Lung/*immunology/metabolism
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/*administration & dosage/immunology
MH  - Pneumonia/blood/immunology/*prevention & control
MH  - Respiratory Hypersensitivity/blood/immunology/*prevention & control
MH  - Skin/*immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
OTO - NOTNLM
OT  - aging
OT  - allergy
OT  - epicutaneous sensitization
OT  - pulmonary inflammation
EDAT- 2017/05/04 06:00
MHDA- 2019/06/27 06:00
CRDT- 2017/05/04 06:00
PHST- 2016/08/29 00:00 [received]
PHST- 2017/04/20 00:00 [revised]
PHST- 2017/04/22 00:00 [accepted]
PHST- 2017/05/04 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2017/05/04 06:00 [entrez]
AID - 10.1111/1440-1681.12778 [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 2017 Dec;44 Suppl 1:64-69. doi: 
      10.1111/1440-1681.12778. Epub 2017 Oct 19.