PMID- 28468753
OWN - NLM
STAT- MEDLINE
DCOM- 20170727
LR  - 20210109
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 36
IP  - 14
DP  - 2017 Jul 14
TI  - Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis 
      for 70S dimerization.
PG  - 2061-2072
LID - 10.15252/embj.201696189 [doi]
AB  - Under stress conditions, such as nutrient deprivation, bacteria enter into a 
      hibernation stage, which is characterized by the appearance of 100S ribosomal 
      particles. In Escherichia coli, dimerization of 70S ribosomes into 100S requires 
      the action of the ribosome modulation factor (RMF) and the hibernation-promoting 
      factor (HPF). Most other bacteria lack RMF and instead contain a long form HPF 
      (LHPF), which is necessary and sufficient for 100S formation. While some 
      structural information exists as to how RMF and HPF mediate formation of E. coli 
      100S (Ec100S), structural insight into 100S formation by LHPF has so far been 
      lacking. Here we present a cryo-EM structure of the Bacillus subtilis hibernating 
      100S (Bs100S), revealing that the C-terminal domain (CTD) of the LHPF occupies a 
      site on the 30S platform distinct from RMF Moreover, unlike RMF, the BsHPF-CTD is 
      directly involved in forming the dimer interface, thereby illustrating the 
      divergent mechanisms by which 100S formation is mediated in the majority of 
      bacteria that contain LHPF, compared to some gamma-proteobacteria, such as E. coli.
CI  - (c) 2017 The Authors.
FAU - Beckert, Bertrand
AU  - Beckert B
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany.
FAU - Abdelshahid, Maha
AU  - Abdelshahid M
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany.
FAU - Schafer, Heinrich
AU  - Schafer H
AD  - Naturwissenschaftliche Fakultat, Institut fur Mikrobiologie, Leibniz Universitat 
      Hannover, Hannover, Germany.
FAU - Steinchen, Wieland
AU  - Steinchen W
AD  - LOEWE Center for Synthetic Microbiology and Faculty of Chemistry, Philipps 
      University Marburg, Marburg, Germany.
FAU - Arenz, Stefan
AU  - Arenz S
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany.
FAU - Berninghausen, Otto
AU  - Berninghausen O
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany.
FAU - Beckmann, Roland
AU  - Beckmann R
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany.
FAU - Bange, Gert
AU  - Bange G
AUID- ORCID: 0000-0002-7826-0932
AD  - LOEWE Center for Synthetic Microbiology and Faculty of Chemistry, Philipps 
      University Marburg, Marburg, Germany gert.bange@synmikro.uni-marburg.de 
      turgay@ifmb.uni-hannover.de Daniel.Wilson@chemie.uni-hamburg.de.
FAU - Turgay, Kursad
AU  - Turgay K
AUID- ORCID: 0000-0002-8959-492X
AD  - Naturwissenschaftliche Fakultat, Institut fur Mikrobiologie, Leibniz Universitat 
      Hannover, Hannover, Germany gert.bange@synmikro.uni-marburg.de 
      turgay@ifmb.uni-hannover.de Daniel.Wilson@chemie.uni-hamburg.de.
FAU - Wilson, Daniel N
AU  - Wilson DN
AUID- ORCID: 0000-0003-3816-3828
AD  - Gene Center, Department for Biochemistry and Center for integrated Protein 
      Science Munich (CiPSM), University of Munich, Munich, Germany 
      gert.bange@synmikro.uni-marburg.de turgay@ifmb.uni-hannover.de 
      Daniel.Wilson@chemie.uni-hamburg.de.
AD  - Institute for Biochemistry and Molecular Biology, University of Hamburg, Hamburg, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170503
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Bacterial Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (yvyD protein, Bacillus subtilis)
SB  - IM
CIN - EMBO J. 2017 Jul 14;36(14 ):1996-1998. PMID: 28673933
MH  - Bacillus subtilis/*metabolism/*ultrastructure
MH  - Bacterial Proteins/*metabolism
MH  - Cryoelectron Microscopy
MH  - *Dimerization
MH  - Heat-Shock Proteins/*metabolism
MH  - Models, Molecular
MH  - Protein Binding
MH  - Ribosomes/*metabolism/*ultrastructure
PMC - PMC5509997
OTO - NOTNLM
OT  - HPF
OT  - RMF
OT  - cryo-EM
OT  - hibernation
OT  - translation
EDAT- 2017/05/05 06:00
MHDA- 2017/07/28 06:00
PMCR- 2018/07/14
CRDT- 2017/05/05 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/03/26 00:00 [revised]
PHST- 2017/03/29 00:00 [accepted]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2017/07/28 06:00 [medline]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2018/07/14 00:00 [pmc-release]
AID - embj.201696189 [pii]
AID - EMBJ201696189 [pii]
AID - 10.15252/embj.201696189 [doi]
PST - ppublish
SO  - EMBO J. 2017 Jul 14;36(14):2061-2072. doi: 10.15252/embj.201696189. Epub 2017 May 
      3.