PMID- 28469184
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20220311
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 May 3
TI  - Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways 
      Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry.
PG  - 1423
LID - 10.1038/s41598-017-01435-7 [doi]
LID - 1423
AB  - Metabolites containing amino groups cover multiple pathways and play important 
      roles in redox homeostasis and biosyntheses of proteins, nucleotides and 
      neurotransmitters. Here, we report a new method for simultaneous quantification 
      of 124 such metabolites. This is achieved by derivatization-assisted sensitivity 
      enhancement with 5-aminoisoquinolyl-N-hydroxysuccinimidyl carbamate (5-AIQC) 
      followed with comprehensive analysis using ultra-high performance liquid 
      chromatography and electrospray ionization tandem mass spectrometry 
      (UHPLC-MS/MS). In an one-pot manner, this quantification method enables 
      simultaneous coverage of 20 important metabolic pathways including protein 
      biosynthesis/degradation, biosyntheses of catecholamines, arginine and 
      glutathione, metabolisms of homocysteine, taurine-hypotaurine etc. Compared with 
      the reported ones, this method is capable of simultaneously quantifying thiols, 
      disulfides and other oxidation-prone analytes in a single run and suitable for 
      quantifying aromatic amino metabolites. This method is also much more sensitive 
      for all tested metabolites with LODs well below 50 fmol (at sub-fmol for most 
      tested analytes) and shows good precision for retention time and quantitation 
      with inter-day and intra-day relative standard deviations (RSDs) below 15% and 
      good recovery from renal cancer tissue, rat urine and plasma. The method was 
      further applied to quantify the amino metabolites in silkworm hemolymph from 
      multiple developmental stages showing its applicability in metabolomics and 
      perhaps some clinical chemistry studies.
FAU - Wang, Jin
AU  - Wang J
AD  - School of Life Science and Technology, Huazhong University of Science and 
      Technology, Wuhan, 430074, China.
AD  - State Key Laboratory of Genetic Engineering, Zhongshan Hospital and School of 
      Life Sciences, Fudan University, Shanghai International Centre for Molecular 
      Phenomics, Collaborative Innovation Center for Genetics and Development, 
      Shanghai, 200438, China.
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key 
      Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National 
      Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and 
      Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
FAU - Zhou, Lihong
AU  - Zhou L
AD  - School of Life Science and Technology, Huazhong University of Science and 
      Technology, Wuhan, 430074, China.
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key 
      Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National 
      Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and 
      Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
FAU - Lei, Hehua
AU  - Lei H
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key 
      Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National 
      Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and 
      Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
FAU - Hao, Fuhua
AU  - Hao F
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key 
      Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National 
      Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and 
      Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
FAU - Liu, Xin
AU  - Liu X
AD  - School of Life Science and Technology, Huazhong University of Science and 
      Technology, Wuhan, 430074, China.
FAU - Wang, Yulan
AU  - Wang Y
AD  - CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key 
      Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National 
      Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and 
      Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases, Zhejiang University, Hangzhou, 310058, China.
FAU - Tang, Huiru
AU  - Tang H
AD  - State Key Laboratory of Genetic Engineering, Zhongshan Hospital and School of 
      Life Sciences, Fudan University, Shanghai International Centre for Molecular 
      Phenomics, Collaborative Innovation Center for Genetics and Development, 
      Shanghai, 200438, China. huiru_tang@fudan.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170503
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - *Chromatography, Liquid
MH  - Humans
MH  - Metabolic Networks and Pathways
MH  - Metabolomics/*methods
MH  - Rats, Wistar
MH  - Reproducibility of Results
MH  - *Tandem Mass Spectrometry
PMC - PMC5431165
COIS- The authors declare that they have no competing interests.
EDAT- 2017/05/05 06:00
MHDA- 2018/12/12 06:00
PMCR- 2017/05/03
CRDT- 2017/05/05 06:00
PHST- 2016/11/21 00:00 [received]
PHST- 2017/03/28 00:00 [accepted]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/05/03 00:00 [pmc-release]
AID - 10.1038/s41598-017-01435-7 [pii]
AID - 1435 [pii]
AID - 10.1038/s41598-017-01435-7 [doi]
PST - epublish
SO  - Sci Rep. 2017 May 3;7(1):1423. doi: 10.1038/s41598-017-01435-7.