PMID- 28469695
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20181113
IS  - 1742-6405 (Electronic)
IS  - 1742-6405 (Linking)
VI  - 14
DP  - 2017
TI  - Molecular diagnosis of central nervous system opportunistic infections and 
      mortality in HIV-infected adults in Central China.
PG  - 24
LID - 10.1186/s12981-017-0150-2 [doi]
LID - 24
AB  - BACKGROUND: CSF PCR is the standard diagnostic technique used in resource-rich 
      settings to detect pathogens of the CNS infection. However, it is not currently 
      used for routine CSF testing in China. Knowledge of CNS opportunistic infections 
      among people living with HIV in China is limited. METHODS: Intensive 
      cerebrospiral fluid (CSF) testing was performed to evaluate for bacterial, viral 
      and fungal etiologies. Pathogen-specific primers were used to detect DNA from 
      cytomegalovirus (CMV), herpes simplex virus (HSV), varicella-zoster virus (VZV), 
      Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6) and John Cunningham virus 
      (JCV) via real-time polymerase chain reaction (PCR). RESULTS: Cryptococcal 
      meningitis accounted for 63.0% (34 of 54) of all causes of meningitis, 13.0% 
      (7/54) for TB, 9.3% (5/54) for Toxoplasma gondii. Of 54 samples sent for viral 
      PCR, 31.5% (17/54) were positive, 12 (22.2%) for CMV, 2 (3.7%) for VZV, 1 (1.9%) 
      for EBV, 1 (1.9%) for HHV-6 and 1 (1.9%) for JCV. No patient was positive for 
      HSV. Pathogen-based treatment and high GCS score tended to have a lower mortality 
      rate, whereas patients with multiple pathogens infection, seizures or 
      intracranial hypertension showed higher odds of death. CONCLUSION: CNS OIs are 
      frequent and multiple pathogens often coexist in CSF. Cryptococcal meningitis is 
      the most prevalent CNS disorders among AIDS. The utility of molecular diagnostics 
      for pathogen identification combined with the knowledge provided by the 
      investigation may improve the diagnosis of AIDS related OIs in resource-limited 
      developing countries, but the cost-efficacy remains to be further evaluated.
FAU - Yang, Rongrong
AU  - Yang R
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Zhang, Hong
AU  - Zhang H
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Xiong, Yong
AU  - Xiong Y
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Gui, Xien
AU  - Gui X
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Zhang, Yongxi
AU  - Zhang Y
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Deng, Liping
AU  - Deng L
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Gao, Shicheng
AU  - Gao S
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Luo, Mingqi
AU  - Luo M
AD  - Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 
      Donghu Road, Wuhan, 430071 China. GRID: grid.413247.7
FAU - Hou, Wei
AU  - Hou W
AD  - Basic Medical College of Wuhan University, Wuhan, China. ISNI: 0000 0001 2331 
      6153. GRID: grid.49470.3e
FAU - Guo, Deyin
AU  - Guo D
AD  - Basic Medical College of Wuhan University, Wuhan, China. ISNI: 0000 0001 2331 
      6153. GRID: grid.49470.3e
LA  - eng
PT  - Journal Article
DEP - 20170502
PL  - England
TA  - AIDS Res Ther
JT  - AIDS research and therapy
JID - 101237921
SB  - IM
MH  - AIDS-Related Opportunistic Infections/*diagnosis/epidemiology/*mortality
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bacteria/classification/isolation & purification
MH  - Central Nervous System Infections/*diagnosis/epidemiology/*mortality
MH  - Cerebrospinal Fluid/microbiology/virology
MH  - China/epidemiology
MH  - Female
MH  - Fungi/classification/isolation & purification
MH  - HIV Infections/*complications
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Viruses/classification/isolation & purification
MH  - Young Adult
PMC - PMC5414136
OTO - NOTNLM
OT  - AIDS
OT  - Central nervous system diseases
OT  - Cerebrospinal fluid
EDAT- 2017/05/05 06:00
MHDA- 2018/03/20 06:00
PMCR- 2017/05/02
CRDT- 2017/05/05 06:00
PHST- 2017/01/18 00:00 [received]
PHST- 2017/04/20 00:00 [accepted]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2017/05/02 00:00 [pmc-release]
AID - 150 [pii]
AID - 10.1186/s12981-017-0150-2 [doi]
PST - epublish
SO  - AIDS Res Ther. 2017 May 2;14:24. doi: 10.1186/s12981-017-0150-2. eCollection 
      2017.