PMID- 28469779
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 9
IP  - 4
DP  - 2017
TI  - Analysis of differentially expressed microRNAs in MEN1 parathyroid adenomas.
PG  - 1743-1753
AB  - Multiple Endocrine Neoplasia type 1 (MEN1) syndrome is a rare complex 
      tumor-predisposing hereditary disorder, inherited in an autosomal dominant manner 
      (OMIM 131100). MEN1 is characterized by tumors of the parathyroids, the 
      neuroendocrine cells of the gastro-entero-pancreatic tract, and the anterior 
      pituitary. The molecular mechanisms that control parathyroid tumorigenesis are 
      still poorly understood. Here we studied the global microRNAs (miRNAs) expression 
      profile in MEN1 parathyroid adenomas to understand the role of these regulatory 
      factors in MEN1 parathyroid tumorigenesis. miRNA arrays containing 1890 human 
      miRNAs were used to profile seven different MEN1 parathyroid adenomas (four 
      presenting somatic loss of heterozygosity (LOH) at 11q13 and three still 
      retaining one wild type copy of the MEN1 gene). Eight miRNAs in non-LOH MEN1 
      parathyroid adenomas and two miRNAs in LOH MEN1 parathyroid adenomas resulted to 
      be differentially expressed, with a significant fold change, with respect to the 
      control pool. Six microRNAs also resulted to be differentially expressed between 
      LOH MEN1 parathyroid adenomas and non-LOH MEN1 parathyroid adenomas. 
      Significantly differentially expressed miRNAs were all validated by SYBR green 
      real-time quantitative RT-PCR. Pearson correlation coefficient indicated 
      miR-4258, miR-664 and miR-1301 as the most significant miRNAs. In silico 
      target-prediction and network analysis showed miR-664 and miR-1301 as organized 
      in predicted GRNs with genes interested in parathyroid adenomas and carcinomas. 
      In conclusion, our study identified three new miRNAs involved in the MEN1 
      parathyroid neoplasia, directly targeting genes associated with the development 
      of different inheritable forms of parathyroid tumors. These identified miRNAs 
      could be revealed as prognostic and diagnostic biomarkers for parathyroid tumors 
      to improve the diagnosis of MEN1 neoplasia and other syndromes.
FAU - Luzi, Ettore
AU  - Luzi E
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
FAU - Ciuffi, Simone
AU  - Ciuffi S
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
FAU - Marini, Francesca
AU  - Marini F
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
FAU - Mavilia, Carmelo
AU  - Mavilia C
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
FAU - Galli, Gianna
AU  - Galli G
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
FAU - Brandi, Maria Luisa
AU  - Brandi ML
AD  - Unit of Bone and Mineral Metabolic Diseases, Department of Surgery and 
      Translational Medicine, University of FlorenceFlorence, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170415
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5411922
OTO - NOTNLM
OT  - MEN1
OT  - diagnostic biomarkers
OT  - gene regulatory networks
OT  - microRNAs
OT  - neoplasia
OT  - parathyroid adenomas
OT  - primary hyperparathyroidism
COIS- None.
EDAT- 2017/05/05 06:00
MHDA- 2017/05/05 06:01
PMCR- 2017/04/15
CRDT- 2017/05/05 06:00
PHST- 2016/10/04 00:00 [received]
PHST- 2017/02/09 00:00 [accepted]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2017/05/05 06:01 [medline]
PHST- 2017/04/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2017 Apr 15;9(4):1743-1753. eCollection 2017.