PMID- 28470729
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20181113
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 189
IP  - 3
DP  - 2017 Sep
TI  - Airway epithelial cells enhance the immunogenicity of human myeloid dendritic 
      cells under steady state.
PG  - 279-289
LID - 10.1111/cei.12983 [doi]
AB  - Dendritic cells (DCs) and airway epithelial cells (AECs) are in close proximity, 
      and AECs secrete factors such as retinoic acid which induce tolerance in DCs at 
      homeostasis. However, the question remains as to how DCs in the lung are able to 
      respond to pathogens in the immunosuppressive environment. Using an in vitro 
      human myeloid DC (mDC)-AEC co-culture system, we demonstrate that AECs induced 
      several gene changes in the mDCs cultured with AECs compared to the mDCs not 
      cultured with AECs. Analysis revealed that several chemokine genes were altered. 
      These chemokine genes could serve to attract neutrophils, natural killer (NK) T 
      as well as T helper type 1 (Th1)/Th2 cells to the airways. Genes priming lipid 
      and major histocompatibility complex (MHC) class II antigen presentation were 
      also up-regulated, along with certain anti-microbial protein genes. In addition, 
      the expression and function of pathogen-sensing Toll-like receptors (TLRs) as 
      well as Nod-like receptors (NLRs) and their downstream signalling molecules were 
      up-regulated in mDCs cultured with AECs. Moreover, murine mucosal DCs from the 
      lung expressed significantly higher levels of TLRs and NLRs compared to 
      peripheral DCs from the spleen. These results indicate that AECs prime mDCs to 
      enhance their immunogenicity, which could be one of the mechanisms that 
      compensates for the immunosuppressive mucosal environment.
CI  - (c) 2017 British Society for Immunology.
FAU - Agrawal, S
AU  - Agrawal S
AD  - Division of Basic and Clinical Immunology, Department of Medicine, Irvine, CA, 
      USA.
FAU - Srivastava, R
AU  - Srivastava R
AD  - Laboratory of Cellular and Molecular Immunology, Gavin Herbert Eye Institute, 
      Irvine, CA, USA.
FAU - Rahmatpanah, F
AU  - Rahmatpanah F
AD  - Department of pathology, University of California, Irvine, Irvine, CA, USA.
FAU - Madiraju, C
AU  - Madiraju C
AD  - Division of Basic and Clinical Immunology, Department of Medicine, Irvine, CA, 
      USA.
FAU - BenMohamed, L
AU  - BenMohamed L
AD  - Laboratory of Cellular and Molecular Immunology, Gavin Herbert Eye Institute, 
      Irvine, CA, USA.
FAU - Agrawal, A
AU  - Agrawal A
AUID- ORCID: 0000-0002-3323-9152
AD  - Division of Basic and Clinical Immunology, Department of Medicine, Irvine, CA, 
      USA.
LA  - eng
GR  - P30 CA062203/CA/NCI NIH HHS/United States
GR  - R01 AG045216/AG/NIA NIH HHS/United States
GR  - UL1 TR000153/TR/NCATS NIH HHS/United States
GR  - UL1 TR001414/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170522
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (NLR Proteins)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Bronchi/*cytology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chemokines/genetics/immunology
MH  - Coculture Techniques
MH  - Cytokines/genetics/immunology
MH  - Dendritic Cells/*immunology
MH  - Epithelial Cells/*immunology
MH  - Genes, MHC Class II
MH  - Humans
MH  - Mice
MH  - Myeloid Cells/*immunology
MH  - NLR Proteins/genetics/metabolism
MH  - Toll-Like Receptors/genetics/immunology
PMC - PMC5543474
OTO - NOTNLM
OT  - bronchial epithelial cells
OT  - chemokines
OT  - myeloid dendritic cells
OT  - pathogen recognition receptors
EDAT- 2017/05/05 06:00
MHDA- 2017/10/17 06:00
PMCR- 2018/09/01
CRDT- 2017/05/05 06:00
PHST- 2017/04/25 00:00 [accepted]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2018/09/01 00:00 [pmc-release]
AID - CEI12983 [pii]
AID - 10.1111/cei.12983 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2017 Sep;189(3):279-289. doi: 10.1111/cei.12983. Epub 2017 May 
      22.