PMID- 28470860
OWN - NLM
STAT- MEDLINE
DCOM- 20180212
LR  - 20181202
IS  - 1468-1331 (Electronic)
IS  - 1351-5101 (Linking)
VI  - 24
IP  - 6
DP  - 2017 Jun
TI  - Multiple antibody detection in 'seronegative' myasthenia gravis patients.
PG  - 844-850
LID - 10.1111/ene.13300 [doi]
AB  - BACKGROUND AND PURPOSE: Myasthenia gravis (MG) is an autoimmune disease caused by 
      antibody mediated impairment in the neuromuscular junction. Seronegative MG 
      (SNMG) without antibodies against acetylcholine receptor (AChR) and 
      muscle-specific kinase (MuSK) by routine assays accounts for about 20% of all MG 
      patients. METHODS: Plasma from 81 Chinese MG patients previously found to be 
      seronegative was tested by routine assays for AChR and MuSK antibodies. These 
      samples were screened by (i) a novel, highly sensitive radioimmunoassay for AChR 
      antibodies; (ii) cell-based assays for clustered AChR, MuSK and lipoprotein 
      receptor-related protein 4 (LRP4) antibodies; (iii) a radioimmunoassay for titin 
      antibodies. RESULTS: Antibodies to AChR, MuSK, LRP4 and titin were found in 25% 
      (20/81), 4% (3/81), 7% (6/81) and 6% (5/78) of SNMG patients, respectively. In 
      total, 37% of SNMG patients were found to be positive for at least one of the 
      tested antibodies. AChR antibody positive patients had more severe disease (P = 
      0.008) and a trend towards fewer remissions/minimal manifestations than AChR 
      antibody negative patients. The four patients with coexistence of antibodies had 
      more severe disease, whilst the seronegative patients had milder MG (P = 0.015). 
      CONCLUSIONS: Detection of multiple muscle antibodies by more sensitive assays 
      provides additional information in diagnosing and subgrouping of MG and may guide 
      MG treatment.
CI  - (c) 2017 EAN.
FAU - Hong, Y
AU  - Hong Y
AUID- ORCID: 0000-0002-3125-2855
AD  - Department of Clinical Medicine, University of Bergen, Bergen, Norway.
FAU - Zisimopoulou, P
AU  - Zisimopoulou P
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
FAU - Trakas, N
AU  - Trakas N
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
FAU - Karagiorgou, K
AU  - Karagiorgou K
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
AD  - Tzartos NeuroDiagnostics, Athens, Greece.
FAU - Stergiou, C
AU  - Stergiou C
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
AD  - Tzartos NeuroDiagnostics, Athens, Greece.
FAU - Skeie, G O
AU  - Skeie GO
AD  - Department of Neurology, Haukeland University Hospital, Bergen, Norway.
FAU - Hao, H-J
AU  - Hao HJ
AD  - Department of Neurology, Peking University First Hospital, Beijing, China.
FAU - Gao, X
AU  - Gao X
AD  - Department of Neurology, Affiliated Hospital of Qingdao University, Qingdao, 
      China.
FAU - Owe, J F
AU  - Owe JF
AD  - Department of Neurology, Haukeland University Hospital, Bergen, Norway.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Neurology, Affiliated Hospital of Qingdao University, Qingdao, 
      China.
FAU - Yue, Y-X
AU  - Yue YX
AD  - Department of Neurology, Qilu Hospital of Shandong University, Jinan, China.
FAU - Romi, F
AU  - Romi F
AD  - Department of Clinical Medicine, University of Bergen, Bergen, Norway.
AD  - Department of Neurology, Haukeland University Hospital, Bergen, Norway.
FAU - Wang, Q
AU  - Wang Q
AD  - Department of Neurology, Affiliated Hospital of Qingdao University, Qingdao, 
      China.
FAU - Li, H-F
AU  - Li HF
AD  - Department of Neurology, Qilu Hospital of Shandong University, Jinan, China.
FAU - Gilhus, N E
AU  - Gilhus NE
AD  - Department of Clinical Medicine, University of Bergen, Bergen, Norway.
AD  - Department of Neurology, Haukeland University Hospital, Bergen, Norway.
FAU - Tzartos, S J
AU  - Tzartos SJ
AD  - Department of Neurobiology, Hellenic Pasteur Institute, Athens, Greece.
AD  - Tzartos NeuroDiagnostics, Athens, Greece.
AD  - University of Patras, Patras, Greece.
LA  - eng
PT  - Journal Article
DEP - 20170504
PL  - England
TA  - Eur J Neurol
JT  - European journal of neurology
JID - 9506311
RN  - 0 (Autoantibodies)
RN  - 0 (Connectin)
RN  - 0 (LDL-Receptor Related Proteins)
RN  - 0 (LRP4 protein, human)
RN  - 0 (Receptors, Cholinergic)
RN  - 0 (TTN protein, human)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Autoantibodies/*blood
MH  - Connectin/*immunology
MH  - Female
MH  - Humans
MH  - LDL-Receptor Related Proteins/*immunology
MH  - Male
MH  - Middle Aged
MH  - Myasthenia Gravis/blood/*immunology
MH  - Radioimmunoassay
MH  - Receptor Protein-Tyrosine Kinases/*immunology
MH  - Receptors, Cholinergic/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - antibodies
OT  - cell-based assay
OT  - clustered acetylcholine receptor
OT  - lipoprotein receptor-related protein 4
OT  - muscle-specific kinase
OT  - myasthenia gravis
OT  - radioimmunoassay
OT  - titin
EDAT- 2017/05/05 06:00
MHDA- 2018/02/13 06:00
CRDT- 2017/05/05 06:00
PHST- 2016/12/08 00:00 [received]
PHST- 2017/03/23 00:00 [accepted]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2018/02/13 06:00 [medline]
PHST- 2017/05/05 06:00 [entrez]
AID - 10.1111/ene.13300 [doi]
PST - ppublish
SO  - Eur J Neurol. 2017 Jun;24(6):844-850. doi: 10.1111/ene.13300. Epub 2017 May 4.