PMID- 28472452 OWN - NLM STAT- MEDLINE DCOM- 20180326 LR - 20180326 IS - 1945-239X (Electronic) IS - 0021-9665 (Linking) VI - 55 IP - 8 DP - 2017 Sep 1 TI - Determination of MS-222 in Water Samples by Solid-phase Extraction Coupled with Liquid Chromatography/Tandem Mass Spectrometry. PG - 813-817 LID - 10.1093/chromsci/bmx041 [doi] AB - A practical solid-phase extraction (SPE) method coupled with liquid chromatography/tandem mass spectrometry (LC-MS/MS) has been developed for the determination of the fish anesthetic MS-222 in water. Water samples were concentrated and purified using three SPE cartridges of different specifications. Elution curves of MS-222 were constructed using various methanol-water solutions on the different cartridges, and SPE conditions were optimized in accordance with the elution curves. The mobile phase containing methanol and 0.1% formic acid solution with a linear gradient elution was utilized to separate MS-222 on a C18 column. Detection was carried out by a triple-quadrupole mass spectrometry with an electrospray ion source in positive mode. Recoveries of three MS-222 fortified levels of 0.05, 0.5 and 5 mug/L ranged of 82.6-101% with relative standard deviations (RSDs) below 9.36%. The limit of detection (LOD) and limit of quantification (LOQ) of MS-222 were 0.01 mug/L and 0.03 mug/L, respectively. This method was satisfactorily applied to the determination of MS-222 in actual water samples collected from aquatic product transportation vehicles or from the natural water catchments. CI - (c) The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com. FAU - Zhao, Dong-Hao AU - Zhao DH AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Wang, Qiang AU - Wang Q AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Wang, Xu-Feng AU - Wang XF AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Li, Zhi-Guang AU - Li ZG AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Li, Yong-Xian AU - Li YX AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Huang, Ke AU - Huang K AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. FAU - Li, Liu-Dong AU - Li LD AD - Key Lab. of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haizhu District, Guangzhou, Guandong 510300, PR China. LA - eng PT - Journal Article PL - United States TA - J Chromatogr Sci JT - Journal of chromatographic science JID - 0173225 RN - 0 (Aminobenzoates) RN - 0 (Water Pollutants, Chemical) RN - 02591PHL19 (tricaine) RN - Y4S76JWI15 (Methanol) SB - IM MH - Aminobenzoates/*analysis/chemistry/isolation & purification MH - Chromatography, Liquid/*methods MH - Limit of Detection MH - Linear Models MH - Methanol MH - Reproducibility of Results MH - Solid Phase Extraction/*methods MH - Tandem Mass Spectrometry/*methods MH - Water Pollutants, Chemical/*analysis/chemistry/isolation & purification EDAT- 2017/05/05 06:00 MHDA- 2018/03/27 06:00 CRDT- 2017/05/05 06:00 PHST- 2016/02/28 00:00 [received] PHST- 2017/05/05 06:00 [pubmed] PHST- 2018/03/27 06:00 [medline] PHST- 2017/05/05 06:00 [entrez] AID - 3798182 [pii] AID - 10.1093/chromsci/bmx041 [doi] PST - ppublish SO - J Chromatogr Sci. 2017 Sep 1;55(8):813-817. doi: 10.1093/chromsci/bmx041.