PMID- 28472596
OWN - NLM
STAT- MEDLINE
DCOM- 20170908
LR  - 20220311
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 36
IP  - 6
DP  - 2017 Jun
TI  - Reciprocal Regulation Between miR-148a/152 and DNA Methyltransferase 1 Is 
      Associated with Hyperhomocysteinemia-Accelerated Atherosclerosis.
PG  - 462-474
LID - 10.1089/dna.2017.3651 [doi]
AB  - DNA methyltransferase 1 (DNMT1) and miRNAs are both important regulators of gene 
      expression that have been implicated in the pathogenesis of atherosclerosis. This 
      study was designed to elucidate the potential interaction between DNMT1 and 
      miRNAs in the context of hyperhomocysteinemia (HHcy)-related atherosclerosis. In 
      the aorta of ApoE(-/-) mice fed a high methionine diet, increased expression of 
      miR-148a/152, with decreased DNMT1 mRNA and protein levels, was detected. Similar 
      changes were observed in cultured foam cells stimulated with homocysteine. When 
      miR-148a/152 was overexpressed using viral vectors, DNMT1 expression was 
      suppressed, whereas the expression of adipose differentiation-related protein 
      (ADRP) was enhanced, and the contents of total cholesterol (TC) and cholesteryl 
      ester (CE) were increased in cultured foam cells. Conversely, downregulation of 
      miR-148a/152 led to elevated DNMT1 expression, reduced ADRP expression, and 
      lowered contents of TC and CE. The luciferase reporter assay verified that DNMT1 
      is a target gene for miR-148a/152 and overexpression of DNMT1 can partially 
      reverse the miR-148a/152-induced lipid accumulation in foam cells. Meanwhile, we 
      observed that DNMT1 overexpression enhanced DNA methylation and reduced 
      miR-148a/152 expression. Our data showed reciprocal regulation between 
      miR-148a/152 and DNMT1 in foam cells, which likely plays a critical role in 
      HHcy-related atherosclerosis.
FAU - Yang, Anning
AU  - Yang A
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Sun, Yue
AU  - Sun Y
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Gao, Yuan
AU  - Gao Y
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Yang, Songhao
AU  - Yang S
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Mao, Caiyan
AU  - Mao C
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Ding, Ning
AU  - Ding N
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Deng, Mei
AU  - Deng M
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Wang, Yanhua
AU  - Wang Y
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Yang, Xiaoling
AU  - Yang X
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Jia, Yuexia
AU  - Jia Y
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
FAU - Zhang, Huiping
AU  - Zhang H
AD  - 2 Prenatal Diagnosis Center of Ningxia Medical University General Hospital , 
      Yinchuan, China .
FAU - Jiang, Yideng
AU  - Jiang Y
AD  - 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Ningxia Medical University , Yinchuan, China .
LA  - eng
PT  - Journal Article
DEP - 20170504
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Apolipoproteins E)
RN  - 0 (Cholesterol Esters)
RN  - 0 (MIRN152 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn148 microRNA, mouse)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
RN  - EC 2.1.1.37 (Dnmt1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/deficiency/genetics
MH  - Atherosclerosis/*complications/enzymology/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cholesterol/metabolism
MH  - Cholesterol Esters/metabolism
MH  - DNA (Cytosine-5-)-Methyltransferase 1
MH  - DNA (Cytosine-5-)-Methyltransferases/*metabolism
MH  - DNA Methylation/drug effects
MH  - Foam Cells/drug effects/metabolism
MH  - Humans
MH  - Hyperhomocysteinemia/*complications
MH  - Methionine/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics
MH  - Promoter Regions, Genetic/genetics
OTO - NOTNLM
OT  - DNA methylation
OT  - DNMT1
OT  - atherosclerosis
OT  - homocysteine
OT  - miR-148a/152
EDAT- 2017/05/05 06:00
MHDA- 2017/09/09 06:00
CRDT- 2017/05/05 06:00
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2017/09/09 06:00 [medline]
PHST- 2017/05/05 06:00 [entrez]
AID - 10.1089/dna.2017.3651 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2017 Jun;36(6):462-474. doi: 10.1089/dna.2017.3651. Epub 2017 May 
      4.