PMID- 2847351
OWN - NLM
STAT- MEDLINE
DCOM- 19881221
LR  - 20161123
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 60
IP  - 1
DP  - 1988 Aug 30
TI  - An international standard for low molecular weight heparin.
PG  - 1-7
AB  - An international collaborative study has been carried out with the aim of 
      establishing an international standard for low molecular weight (LMW) heparin. 
      Three preparations of LMW heparin were assayed against the International Standard 
      for unfractionated heparin (UFH) by 25 laboratories in 13 countries, using nine 
      different assay methods. The results confirmed previous findings of non-parallel 
      assays, wide interlaboratory variability and differences between methods when LMW 
      heparins are assayed against a UFH standard. Use of one of the LMW heparins as a 
      standard for the other two gave parallel assays and much closer agreement between 
      laboratories. The preparation in ampoules coded 85/600 was selected as likely to 
      give the best agreement with the largest number of LMW heparins; potencies were 
      assigned by taking the mean of all the anti-Xa assays, and the mean of the 
      thrombin and APTT assays, to represent the two major groups of activities. 
      Preparation 85/600 has been established by WHO as the 1st International Standard 
      for LMW heparin, with potencies of 1,680 iu/ampoule by anti-Xa assays and 665 
      iu/ampoule by thrombin inhibition and APTT assays.
FAU - Barrowcliffe, T W
AU  - Barrowcliffe TW
AD  - National Institute for Biological Standards and Control, South Mimms, Herts., UK.
FAU - Curtis, A D
AU  - Curtis AD
FAU - Johnson, E A
AU  - Johnson EA
FAU - Thomas, D P
AU  - Thomas DP
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9000-94-6 (Antithrombin III)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 3.4.21.6 (Factor Xa)
SB  - IM
MH  - Antithrombin III/isolation & purification
MH  - Europe
MH  - Factor Xa
MH  - Heparin, Low-Molecular-Weight/*standards
MH  - Humans
MH  - Pharmacopoeias as Topic
MH  - Reference Standards
MH  - Serine Endopeptidases/immunology
MH  - Thrombin/antagonists & inhibitors
MH  - United Kingdom
MH  - United States
EDAT- 1988/08/30 00:00
MHDA- 1988/08/30 00:01
CRDT- 1988/08/30 00:00
PHST- 1988/08/30 00:00 [pubmed]
PHST- 1988/08/30 00:01 [medline]
PHST- 1988/08/30 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1988 Aug 30;60(1):1-7.