PMID- 2847546
OWN - NLM
STAT- MEDLINE
DCOM- 19881214
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 255
IP  - 5 Pt 2
DP  - 1988 Nov
TI  - Effect of vasopressin on intracellular [Ca] and Na transport in cultured toad 
      bladder cells.
PG  - F1015-24
AB  - Intracellular free [Ca] [( Ca]i) and transepithelial sodium transport were 
      measured simultaneously in cultured toad bladder cells grown on collagen-coated 
      filters. [Ca]i was measured with fura-2 and fluorescence microscopy while sodium 
      transport was measured as the short-circuit current (Isc) with a voltage clamp. 
      Following stimulation with vasopressin [Ca]i and Isc rose in parallel to maximal 
      values within 10 min. [Ca]i increased from 65 +/- 5 to 123 +/- 12 nM and Isc, 
      from 11 +/- 3 to 25 +/- 6 microA (n = 4). The vasopressin-induced rise in [Ca]i 
      correlated significantly with the increase in Isc, suggesting that the rise in 
      [Ca]i might be necessary for the increase in Isc. If so, then adenosine 
      3',5'-cyclic monophosphate (cAMP), which mimics the natriferic action of 
      vasopressin, should also increase [Ca]i. Although cAMP increased [Ca]i to a peak 
      value of 32 +/- 13% (P less than 0.05) above control at 10 min, the rise in Isc 
      did not parallel the increase in [Ca]i. Isc peaked instead at 20 min, rising to 
      114 +/- 25% (P less than 0.05) over control, during which time [Ca]i returned to 
      base line. This result suggested that a steady state increase in [Ca]i was not 
      necessary for the natriferic action of cAMP. This notion was confirmed in 
      experiments in which the vasopressin-induced increase in [Ca]i was prevented by 
      bathing the tissue in a low-[Ca] buffer. Under these conditions, Isc increased 37 
      +/- 9% above control (P less than 0.05, n = 4) even though [Ca]i remained largely 
      unchanged. Our results suggest that although vasopressin increases [Ca]i in toad 
      bladder cells, the rise in [Ca]i does not seem to play a role in the natriferic 
      response. These experiments also demonstrate the utility of making simultaneous 
      measurements of ion transport and [Ca]i, which allow direct examination of 
      calcium's role in mediating ion transport.
FAU - Wong, S M
AU  - Wong SM
AD  - Department of Medicine, Columbia College of Physicians and Surgeons, New York, 
      New York 10032.
FAU - Chase, H S Jr
AU  - Chase HS Jr
LA  - eng
GR  - R01 DK-39154/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Benzofurans)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Fluorescent Dyes)
RN  - 11000-17-2 (Vasopressins)
RN  - 57818-92-5 (8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate)
RN  - 632XD903SP (Gallic Acid)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - 9NEZ333N27 (Sodium)
RN  - E0399OZS9N (Cyclic AMP)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Amiloride/pharmacology
MH  - Animals
MH  - Benzofurans
MH  - Biological Transport/drug effects
MH  - Bufonidae
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers
MH  - Cells, Cultured
MH  - Cyclic AMP/pharmacology
MH  - Electric Conductivity
MH  - Epithelium/metabolism
MH  - Fluorescent Dyes
MH  - Fura-2
MH  - Gallic Acid/analogs & derivatives/pharmacology
MH  - Microscopy, Fluorescence
MH  - Sodium/*metabolism
MH  - Urinary Bladder/drug effects/*metabolism
MH  - Vasopressins/*pharmacology
EDAT- 1988/11/11 19:15
MHDA- 1988/11/11 19:16
CRDT- 1988/11/11 19:15
PHST- 1988/11/11 19:15 [pubmed]
PHST- 1988/11/11 19:16 [medline]
PHST- 1988/11/11 19:15 [entrez]
AID - 10.1152/ajprenal.1988.255.5.F1015 [doi]
PST - ppublish
SO  - Am J Physiol. 1988 Nov;255(5 Pt 2):F1015-24. doi: 
      10.1152/ajprenal.1988.255.5.F1015.