PMID- 28476808
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20210208
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 5
DP  - 2017 May
TI  - Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker 
      in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.
PG  - 2407-2415
AB  - BACKGROUND: The deregulation of the cell cycle is crucial for the processes of 
      carcinogenesis and tumor progression. The cell cycle is under the control of 
      cyclin-dependent kinases (CDKs) and their inhibitors (CDKIs). Proteins p16 and 
      p27 are CDKIs, and their altered expression has been noticed in many types of 
      cancers. The aim of this study has been to compare the expression levels of these 
      proteins in laryngeal papillomas and carcinomas in order to confirm their 
      predictive value in cancer development. MATERIALS AND METHODS: Eighty-three cases 
      of laryngeal lesions were analyzed: cancers (51) and papillomas (32). 
      Immunohistochemical (IHC) reactions were performed in order to detect the 
      expression levels of p16, p27 and Ki-67. Immunofluorescence (IF) and western blot 
      were performed on HEp-2 cell line. RESULTS: The expression of p16 and p27 was 
      decreased in cancer cells in comparison to papillomas. Additionally, we observed 
      cytoplasmic expression of p16 in cancers and lack of such expression in 
      papillomas. The same pattern of expression of p16 was also detected by IF and 
      western blot analysis in HEp-2 cell line. The expression of Ki-67 protein 
      revealed no significant differences in the case of papilloma compared with 
      cancer, however a significant correlation between tumor grade and the nuclear 
      expression of Ki-67 was observed. CONCLUSION: There is a necessity in finding 
      biomarkers, which would predict the risk level of malignant transformation. Our 
      study has confirmed that altered expressions of the p16 and p27 proteins might be 
      useful biomarkers in the progression of laryngeal squamous cell carcinomas 
      (LSCC).
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Ciesielska, Urszula
AU  - Ciesielska U
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland urszula.ciesielska@umed.wroc.pl.
FAU - Zatonski, Tomasz
AU  - Zatonski T
AD  - Department of Otolaryngology, Head and Neck Surgery, Wroclaw Medical University, 
      Wroclaw, Poland.
FAU - Nowinska, Katarzyna
AU  - Nowinska K
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Ratajczak-Wielgomas, Katarzyna
AU  - Ratajczak-Wielgomas K
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Grzegrzolka, Jedrzej
AU  - Grzegrzolka J
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Piotrowska, Aleksandra
AU  - Piotrowska A
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Olbromski, Mateusz
AU  - Olbromski M
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Pula, Bartosz
AU  - Pula B
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Podhorska-Okolow, Marzenna
AU  - Podhorska-Okolow M
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AD  - Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, 
      Poland.
AD  - Department of Physiotherapy, University School of Physical Education, Wroclaw, 
      Poland.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Ki-67 Antigen)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Child
MH  - Child, Preschool
MH  - Cyclin-Dependent Kinase Inhibitor p16/*metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p27/*metabolism
MH  - Cytoplasm/metabolism
MH  - Female
MH  - Humans
MH  - Ki-67 Antigen/*metabolism
MH  - Laryngeal Neoplasms/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Papilloma/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Laryngeal Squamous Cell Carcinoma
OT  - Papilloma
OT  - p16
OT  - p27
EDAT- 2017/05/10 06:00
MHDA- 2017/08/15 06:00
CRDT- 2017/05/07 06:00
PHST- 2017/03/04 00:00 [received]
PHST- 2017/03/23 00:00 [revised]
PHST- 2017/03/24 00:00 [accepted]
PHST- 2017/05/07 06:00 [entrez]
PHST- 2017/05/10 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
AID - 37/5/2407 [pii]
AID - 10.21873/anticanres.11580 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 May;37(5):2407-2415. doi: 10.21873/anticanres.11580.