PMID- 28482799
OWN - NLM
STAT- MEDLINE
DCOM- 20171218
LR  - 20201209
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 18
IP  - 1
DP  - 2017 May 8
TI  - No evidence for a bovine mastitis Escherichia coli pathotype.
PG  - 359
LID - 10.1186/s12864-017-3739-x [doi]
LID - 359
AB  - BACKGROUND: Escherichia coli bovine mastitis is a disease of significant economic 
      importance in the dairy industry. Molecular characterization of 
      mastitis-associated E. coli (MAEC) did not result in the identification of common 
      traits. Nevertheless, a mammary pathogenic E. coli (MPEC) pathotype has been 
      proposed suggesting virulence traits that differentiate MAEC from commensal E. 
      coli. The present study was designed to investigate the MPEC pathotype hypothesis 
      by comparing the genomes of MAEC and commensal bovine E. coli. RESULTS: We 
      sequenced the genomes of eight E. coli isolated from bovine mastitis cases and 
      six fecal commensal isolates from udder-healthy cows. We analyzed the 
      phylogenetic history of bovine E. coli genomes by supplementing this strain panel 
      with eleven bovine-associated E. coli from public databases. The majority of the 
      isolates originate from phylogroups A and B1, but neither MAEC nor commensal 
      strains could be unambiguously distinguished by phylogenetic lineage. The gene 
      content of both MAEC and commensal strains is highly diverse and dominated by 
      their phylogenetic background. Although individual strains carry some typical E. 
      coli virulence-associated genes, no traits important for pathogenicity could be 
      specifically attributed to MAEC. Instead, both commensal strains and MAEC have 
      very few gene families enriched in either pathotype. Only the aerobactin 
      siderophore gene cluster was enriched in commensal E. coli within our strain 
      panel. CONCLUSIONS: This is the first characterization of a phylogenetically 
      diverse strain panel including several MAEC and commensal isolates. With our 
      comparative genomics approach we could not confirm previous studies that argue 
      for a positive selection of specific traits enabling MAEC to elicit bovine 
      mastitis. Instead, MAEC are facultative and opportunistic pathogens recruited 
      from the highly diverse bovine gastrointestinal microbiota. Virulence-associated 
      genes implicated in mastitis are a by-product of commensalism with the primary 
      function to enhance fitness in the bovine gastrointestinal tract. Therefore, we 
      put the definition of the MPEC pathotype into question and suggest to designate 
      corresponding isolates as MAEC.
FAU - Leimbach, Andreas
AU  - Leimbach A
AUID- ORCID: 0000-0001-9077-1041
AD  - Institute of Hygiene, University of Munster, Mendelstrasse 7, 48149, Munster, 
      Germany. aleimba@gmx.de.
AD  - Department of Genomic and Applied Microbiology, Gottingen Genomics Laboratory, 
      Institute of Microbiology and Genetics, Georg-August-University of Gottingen, 
      Gottingen, Germany. aleimba@gmx.de.
AD  - Institute for Molecular Infection Biology, Julius-Maximilians-University of 
      Wurzburg, Wurzburg, Germany. aleimba@gmx.de.
FAU - Poehlein, Anja
AU  - Poehlein A
AUID- ORCID: 0000-0002-2473-6202
AD  - Department of Genomic and Applied Microbiology, Gottingen Genomics Laboratory, 
      Institute of Microbiology and Genetics, Georg-August-University of Gottingen, 
      Gottingen, Germany.
FAU - Vollmers, John
AU  - Vollmers J
AUID- ORCID: 0000-0001-5980-5178
AD  - Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, 
      Braunschweig, Germany.
FAU - Gorlich, Dennis
AU  - Gorlich D
AUID- ORCID: 0000-0002-2574-9419
AD  - Institute of Biostatistics and Clinical Research, University of Munster, Munster, 
      Germany.
FAU - Daniel, Rolf
AU  - Daniel R
AUID- ORCID: 0000-0002-8646-7925
AD  - Department of Genomic and Applied Microbiology, Gottingen Genomics Laboratory, 
      Institute of Microbiology and Genetics, Georg-August-University of Gottingen, 
      Gottingen, Germany.
FAU - Dobrindt, Ulrich
AU  - Dobrindt U
AUID- ORCID: 0000-0001-9949-1898
AD  - Institute of Hygiene, University of Munster, Mendelstrasse 7, 48149, Munster, 
      Germany. dobrindt@uni-muenster.de.
AD  - Institute for Molecular Infection Biology, Julius-Maximilians-University of 
      Wurzburg, Wurzburg, Germany. dobrindt@uni-muenster.de.
LA  - eng
PT  - Journal Article
DEP - 20170508
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Escherichia coli/genetics/*isolation & purification/pathogenicity/*physiology
MH  - Genes, Bacterial/genetics
MH  - Genomic Islands/genetics
MH  - Mastitis, Bovine/*microbiology
MH  - Phylogeny
MH  - Sequence Homology, Nucleic Acid
MH  - Virulence/genetics
PMC - PMC5422975
OTO - NOTNLM
OT  - Bovine mastitis
OT  - Commensals
OT  - Comparative genomics
OT  - E. coli
OT  - Genomic diversity
OT  - Pathotype
OT  - Phylogeny
OT  - Virulence
EDAT- 2017/05/10 06:00
MHDA- 2017/12/19 06:00
PMCR- 2017/05/08
CRDT- 2017/05/10 06:00
PHST- 2016/12/27 00:00 [received]
PHST- 2017/04/27 00:00 [accepted]
PHST- 2017/05/10 06:00 [entrez]
PHST- 2017/05/10 06:00 [pubmed]
PHST- 2017/12/19 06:00 [medline]
PHST- 2017/05/08 00:00 [pmc-release]
AID - 10.1186/s12864-017-3739-x [pii]
AID - 3739 [pii]
AID - 10.1186/s12864-017-3739-x [doi]
PST - epublish
SO  - BMC Genomics. 2017 May 8;18(1):359. doi: 10.1186/s12864-017-3739-x.