PMID- 2848335
OWN - NLM
STAT- MEDLINE
DCOM- 19890109
LR  - 20190727
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 96
IP  - 2
DP  - 1988 Nov
TI  - Dieldrin activates rat neutrophils in vitro.
PG  - 269-78
AB  - Suppression of phagocytic cell function has been proposed as a possible mechanism 
      for the enhanced sensitivity to certain infectious agents exhibited by animals 
      exposed to the organochloride insecticide, dieldrin. In the present study, we 
      examined the effects of dieldrin on superoxide production by glycogen-elicited 
      peritoneal neutrophils (PMNs) from the rat. Dieldrin caused a 
      concentration-dependent increase in superoxide production by PMNs incubated in 
      vitro at 37 degrees C. Superoxide release was increased significantly with 10 
      microM dieldrin and reached a maximum of 17 nmol/10 min/2.0 X 10(6) PMNs at a 
      dieldrin concentration of 35 microM. Preincubation of PMNs for 5 min at room 
      temperature with a barely suprathreshold concentration of either phorbol 
      12-myristate 13-acetate (PMA) or N-formyl-L-methionyl-L-leucyl-L-phenylalanine 
      (FMLP) enhanced dieldrin-stimulated superoxide release by as much as ninefold or 
      threefold, respectively. Maximum enhancement was obtained with 10 microM dieldrin 
      for both PMA and FMLP. Time course studies with PMA-pretreated cells revealed 
      that the rate of superoxide release was dependent on the concentration of 
      dieldrin. Extracellular calcium played an important role in dieldrin-stimulated 
      superoxide release, since PMNs treated with dieldrin in the absence of 
      extracellular calcium did not release superoxide. Also, pretreatment with calcium 
      ionophore A23187 greatly enhanced superoxide release from dieldrin-stimulated 
      PMNs. These results show that dieldrin has a stimulatory effect on superoxide 
      release from rat PMNs in vitro and that this stimulation is dependent on 
      extracellular calcium.
FAU - Hewett, J A
AU  - Hewett JA
AD  - Department of Pharmacology and Toxicology, Michigan State University, East 
      Lansing 48824.
FAU - Roth, R A
AU  - Roth RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 11062-77-4 (Superoxides)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - I0246D2ZS0 (Dieldrin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcimycin/pharmacology
MH  - Calcium/metabolism
MH  - Dieldrin/*pharmacology
MH  - L-Lactate Dehydrogenase/blood
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/enzymology
MH  - Rats
MH  - Superoxides/blood
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1988/11/01 00:00
MHDA- 1988/11/01 00:01
CRDT- 1988/11/01 00:00
PHST- 1988/11/01 00:00 [pubmed]
PHST- 1988/11/01 00:01 [medline]
PHST- 1988/11/01 00:00 [entrez]
AID - 10.1016/0041-008x(88)90086-5 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 1988 Nov;96(2):269-78. doi: 10.1016/0041-008x(88)90086-5.