PMID- 2848449
OWN - NLM
STAT- MEDLINE
DCOM- 19890104
LR  - 20190628
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 267
IP  - 1
DP  - 1988 Nov 15
TI  - Evidence that human rheumatoid synovial matrix metalloproteinase 3 is an 
      endogenous activator of procollagenase.
PG  - 211-6
AB  - The treatment of crude culture medium from human rheumatoid synovial cells with 
      4-aminophenylmercuric acetate (APMA) or trypsin results in the activation of 
      procollagenase. This process was shown to be dependent on the presence of matrix 
      metalloproteinase 3 (MMP-3). MMP-3 can directly activate procollagenase without 
      changing the apparent molecular weight of procollagenase. This activity was 
      accelerated in the presence of APMA. We propose that MMP-3 plays an important 
      role in connective tissue destruction through the activation of procollagenase in 
      addition to its direct action on components of the extracellular matrix.
FAU - Ito, A
AU  - Ito A
AD  - Department of Biochemistry, University of Kansas Medical Center, Kansas City 
      66103.
FAU - Nagase, H
AU  - Nagase H
LA  - eng
GR  - AR 39189/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Enzyme Precursors)
RN  - 6283-24-5 (4-aminophenylmercuriacetate)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.- (procollagenase)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.3 (Microbial Collagenase)
RN  - OSX88361UX (Phenylmercuric Acetate)
SB  - IM
MH  - Arthritis, Rheumatoid/*enzymology
MH  - Cells, Cultured
MH  - *Collagenases
MH  - Enzyme Activation/drug effects
MH  - Enzyme Precursors/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 3
MH  - Metalloendopeptidases/*metabolism
MH  - Microbial Collagenase/*metabolism
MH  - Molecular Weight
MH  - Phenylmercuric Acetate/analogs & derivatives/pharmacology
MH  - Synovial Membrane/*enzymology
MH  - Trypsin/pharmacology
EDAT- 1988/11/15 00:00
MHDA- 1988/11/15 00:01
CRDT- 1988/11/15 00:00
PHST- 1988/11/15 00:00 [pubmed]
PHST- 1988/11/15 00:01 [medline]
PHST- 1988/11/15 00:00 [entrez]
AID - 0003-9861(88)90025-2 [pii]
AID - 10.1016/0003-9861(88)90025-2 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1988 Nov 15;267(1):211-6. doi: 
      10.1016/0003-9861(88)90025-2.