PMID- 28487024
OWN - NLM
STAT- MEDLINE
DCOM- 20180314
LR  - 20181016
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 53
DP  - 2017 May
TI  - Co-localization of microsomal prostaglandin E synthase-1 with cyclooxygenase-1 in 
      layer II of murine placental syncytiotrophoblasts.
PG  - 76-82
LID - S0143-4004(17)30224-2 [pii]
LID - 10.1016/j.placenta.2017.04.002 [doi]
AB  - The placenta is an organ that secretes prostaglandin (PG) E(2) into the 
      fetal-placental circulation to regulate both vascular tone and remodeling of the 
      fetal ductus arteriosus. Placental PGE(2) synthesis might be mediated by 
      microsomal PGE synthase-1 (mPGES-1), in addition to cyclooxygenase (COX) 
      isoforms. Thus, the purpose of this study is to clarify the temporal and spatial 
      expression patterns of mPGES-1, together with COX-1 and COX-2, in murine 
      placenta. We found that mPGES-1 and COX-1 protein levels continuously increased 
      in the placental labyrinth from gestational day (GD) 13.5 to GD19.5, becoming 
      higher than in the decidua or the junctional zone by GD17.5. The PGE(2) level at 
      GD17.5 was also highest in the labyrinth. Immunofluorescence stainings for 
      mPGES-1 and COX-1 in the labyrinth at GD17.5 overlapped and were located on the 
      fetal side of the signals for connexin 26, which forms gap junctions between 
      maternal-facing (SynT-I) and fetal-facing (SynT-II) syncytiotrophoblast layers, 
      and on the maternal side of the signals for glucose transporter 1 on the basal 
      plasma membrane of SynT-II. On the other hand, the signals for COX-2 did not 
      overlap with those for mPGES-1. These results indicate that COX-1 and mPGES-1 are 
      co-localized in murine placental SynT-II, facing the fetal-placental circulation. 
      Therefore, SynT-II could contribute to placental synthesis of PGE(2) for release 
      into the fetal-placental circulation.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Inagaki, Mai
AU  - Inagaki M
AD  - Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 
      105-8512, Japan.
FAU - Nishimura, Tomohiro
AU  - Nishimura T
AD  - Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 
      105-8512, Japan.
FAU - Akanuma, Shin-Ichi
AU  - Akanuma SI
AD  - Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
FAU - Nakanishi, Takeo
AU  - Nakanishi T
AD  - Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and 
      Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
FAU - Tachikawa, Masanori
AU  - Tachikawa M
AD  - Division of Membrane Transport and Drug Targeting, Graduate School of 
      Pharmaceutical Sciences, Tohoku University, 6-3 Aoba Aramaki, Aoba-ku, Sendai, 
      Miyagi 980-8578, Japan.
FAU - Tamai, Ikumi
AU  - Tamai I
AD  - Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and 
      Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
FAU - Hosoya, Ken-Ichi
AU  - Hosoya KI
AD  - Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
FAU - Nakashima, Emi
AU  - Nakashima E
AD  - Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 
      105-8512, Japan.
FAU - Tomi, Masatoshi
AU  - Tomi M
AD  - Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 
      105-8512, Japan. Electronic address: tomi-ms@pha.keio.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170406
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Isoenzymes)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 5.3.99.3 (Prostaglandin-E Synthases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 1/*metabolism
MH  - Cyclooxygenase 2/*metabolism
MH  - Dinoprostone/metabolism
MH  - Female
MH  - Isoenzymes/metabolism
MH  - Mice, Inbred C57BL
MH  - Pregnancy
MH  - Prostaglandin-E Synthases/*metabolism
MH  - Trophoblasts/*enzymology
OTO - NOTNLM
OT  - Cyclooxygenase
OT  - Placenta
OT  - Prostaglandin E synthase
OT  - Prostaglandin E(2)
OT  - Syncytiotrophoblasts
EDAT- 2017/05/11 06:00
MHDA- 2018/03/15 06:00
CRDT- 2017/05/11 06:00
PHST- 2017/02/12 00:00 [received]
PHST- 2017/03/31 00:00 [revised]
PHST- 2017/04/04 00:00 [accepted]
PHST- 2017/05/11 06:00 [entrez]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/03/15 06:00 [medline]
AID - S0143-4004(17)30224-2 [pii]
AID - 10.1016/j.placenta.2017.04.002 [doi]
PST - ppublish
SO  - Placenta. 2017 May;53:76-82. doi: 10.1016/j.placenta.2017.04.002. Epub 2017 Apr 
      6.