PMID- 28487887
OWN - NLM
STAT- MEDLINE
DCOM- 20170807
LR  - 20181113
IS  - 2505-0044 (Electronic)
IS  - 0189-6016 (Print)
IS  - 2505-0044 (Linking)
VI  - 13
IP  - 5
DP  - 2016
TI  - ANTI-INFLAMMATORY ACTIVITY OF EUCALYPTUS SPP. AND PISTASCIA LENTISCUS LEAF 
      EXTRACTS.
PG  - 1-6
LID - 10.21010/ajtcam.v13i5.1 [doi]
AB  - BACKGROUND: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian 
      trees that are traditionally used in folkloric medicine in treating many 
      diseases; leaves of which are thought to have anti-inflammatory, antibacterial 
      and antioxidant effects. The goal of this study is to evaluate the in vitro 
      inhibitory effect of Eucalyptus spp. and Pistascia lentiscus extracts on 
      Lipopolysacaride (LPS)-induced Interlukin-6 (Il-6) and Tumor Necrosis Factor-alpha 
      (TNF-alpha) by polymorphonuclear Cells (PMNCs). MATERIALS AND METHODS: 
      Polymorphonuclear cells were isolated from the whole blood using Histopaque 
      (Ficol-1077) method and then cultured in an enriched Roswell Park Memorial 
      Institute (RBMI) medium. Supernatants' Interlukin-6 (IL-6) and Tumor Necrosis 
      Factor (TNF-alpha) levels were determined 24 hour after LPS stimulation. HPLC was 
      employed to determine the concentration of phenolic compounds in the extracts. 
      The concentrations of TNF-alpha and IL-6 were compared using paired-samples t test. 
      RESULTS: Eucalyptus spp. and Pistascia lentiscus leaves extracts have shown 
      significant reduction in the levels of both Il-6 and TNF-alpha Gallic acid; a strong 
      anti-inflammatory agent was found to be the major phenolic compound in both leaf 
      extracts. However, other anti-inflammatory phenolic compounds were detected in 
      Pitascia lentiscus extract including syringic acid and p-coumaric acid, while 
      chlorogenic acid was detected in Eucalyptus spp. leaf extract. CONCLUSION: 
      Reduction in the levels of Il-6 and TNF-alpha upon the effect of both Eucalyptus spp. 
      and Pistascia lentiscus extract is an indication of their anti-inflammatory 
      effects. Our results may also indicate that the observed anti-inflammatory effect 
      of the above extracts may be due to the presence of gallic acid and other 
      phenolic compounds. List of Abbreviations and Nomenclature: LPS: 
      Lipopolysacaride, Il-6: Interlukin-6, TNF-alpha: Tumor Necrosis Factor-alpha, PMNCs: 
      Polymorphonuclear Cells, HPLC: High Performance Liquid Chromatography, ELISA: 
      Enzyme Linked Immune Sorbent Assay, EDTA: Ethylene Diamine Tetra Acetic acid, 
      PBS: phosphate buffered saline, RPMI: Roswell Park Memorial Institute medium FBS: 
      Fetal Bovine Serum.
FAU - Qabaha, Khaled
AU  - Qabaha K
AD  - Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, 
      Arab American University, Jenin, Palestine.
FAU - Ras, Sari Abu
AU  - Ras SA
AD  - Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, 
      Arab American University, Jenin, Palestine.
FAU - Abbadi, Jehad
AU  - Abbadi J
AD  - Biology Department, Faculty of Science and Technology, Al-Quds University, 
      P.O.Box 20002, East Jerusalem, Palestine.
FAU - Al-Rimawi, Fuad
AU  - Al-Rimawi F
AD  - Chemistry Department, Faculty of Science and Technology, Al-Quds University, P. 
      O. Box 20002, East Jerusalem, Palestine.
LA  - eng
PT  - Journal Article
DEP - 20160812
PL  - Nigeria
TA  - Afr J Tradit Complement Altern Med
JT  - African journal of traditional, complementary, and alternative medicines : AJTCAM
JID - 101232990
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 632XD903SP (Gallic Acid)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Eucalyptus/*chemistry
MH  - Gallic Acid/analysis
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/physiology
MH  - Neutrophils/drug effects
MH  - Phenols/analysis
MH  - Pistacia/*chemistry
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/*chemistry
MH  - Tumor Necrosis Factor-alpha/drug effects
PMC - PMC5416627
OTO - NOTNLM
OT  - Anti-inflammatory
OT  - Eucalyptus spp
OT  - HPLC
OT  - IL-6
OT  - Pistascia lentiscus
OT  - TNF-alpha
EDAT- 2017/05/11 06:00
MHDA- 2017/08/08 06:00
PMCR- 2017/08/12
CRDT- 2017/05/11 06:00
PHST- 2017/05/11 06:00 [entrez]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2017/08/08 06:00 [medline]
PHST- 2017/08/12 00:00 [pmc-release]
AID - AJTCAM-13-1 [pii]
AID - 10.21010/ajtcam.v13i5.1 [doi]
PST - epublish
SO  - Afr J Tradit Complement Altern Med. 2016 Aug 12;13(5):1-6. doi: 
      10.21010/ajtcam.v13i5.1. eCollection 2016.