PMID- 28487950
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Jul
TI  - Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular 
      epithelial cell line.
PG  - 57-62
LID - 10.3892/mmr.2017.6556 [doi]
AB  - Apoptosis and necroptosis occur in renal tubular epithelial cell (RTEC) death in 
      acute kidney injury (AKI), and may be regulated by several methods. The present 
      study identified a protective effect of necrostatin‑1 (Nec‑1) on RTECs via a 
      flow-control-like effect. The results established a hypoxic‑ischemic injury model 
      of rat NRK‑52E RTECs using tumour necrosis factor‑alpha followed by ATP depletion 
      with antimycin A and the pan-caspase pathway blocker, 
      benzyloxycarbonyl-Val-Ala-Asp-fluoro-methylketone. Following pre‑treatment of 
      cells with Nec‑1, cell organelle inflation, fragmentation inhibition and improved 
      cell viability were observed with a parallel reduced expression of 
      microtubule‑associated protein 1A/1B‑light chain 3‑II. Nec‑1 was involved in flow 
      control in the process of cell injury and death. In conclusion, the present study 
      indicated that Nec‑1 provides a protective effect and serves an important role in 
      the prevention of AKI in an NRK‑52E cell model. Further studies will be required 
      to fully investigate the role of Nec‑1 in the development of AKI in vivo.
FAU - Luo, Jialun
AU  - Luo J
AD  - Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
FAU - Tao, Yiming
AU  - Tao Y
AD  - Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
FAU - Liang, Xinling
AU  - Liang X
AD  - Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
FAU - Chen, Yuanhan
AU  - Chen Y
AD  - Department of Nephrology, Guangdong General Hospital, Guangdong Academy of 
      Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
FAU - Jiang, Fen
AU  - Jiang F
AD  - Department of Nephrology, The First Affiliated Hospital of Nanhua University, 
      Hengyang, Hunan 421001, P.R. China.
FAU - Liu, Shuangxin
AU  - Liu S
AD  - Department of Nephrology, Guangdong General Hospital, Guangdong Academy of 
      Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
FAU - Ye, Zhiming
AU  - Ye Z
AD  - Department of Nephrology, Guangdong General Hospital, Guangdong Academy of 
      Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
FAU - Li, Zhilian
AU  - Li Z
AD  - Department of Nephrology, Guangdong General Hospital, Guangdong Academy of 
      Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
FAU - Shi, Wei
AU  - Shi W
AD  - Department of Nephrology, Guangdong General Hospital, Guangdong Academy of 
      Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170509
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Imidazoles)
RN  - 0 (Indoles)
RN  - 0 (Oligopeptides)
RN  - 0 (Protective Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (benzyloxycarbonyl-valyl-alanyl-aspartic acid)
RN  - 0 (necrostatin-1)
RN  - 642-15-9 (Antimycin A)
SB  - IM
MH  - Acute Kidney Injury/drug therapy/etiology/metabolism/pathology
MH  - Animals
MH  - Antimycin A/pharmacology
MH  - Apoptosis/drug effects
MH  - Autophagy
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Epithelial Cells/*drug effects/*metabolism
MH  - Imidazoles/*pharmacology
MH  - Indoles/*pharmacology
MH  - Kidney Tubules, Proximal/*cytology
MH  - Necrosis
MH  - Oligopeptides/pharmacology
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
PMC - PMC5482151
EDAT- 2017/05/11 06:00
MHDA- 2018/03/27 06:00
PMCR- 2017/05/09
CRDT- 2017/05/11 06:00
PHST- 2016/01/19 00:00 [received]
PHST- 2017/02/14 00:00 [accepted]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/05/11 06:00 [entrez]
PHST- 2017/05/09 00:00 [pmc-release]
AID - mmr-16-01-0057 [pii]
AID - 10.3892/mmr.2017.6556 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Jul;16(1):57-62. doi: 10.3892/mmr.2017.6556. Epub 2017 May 9.