PMID- 28487961
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20180226
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary 
      embolism.
PG  - 1580-1588
LID - 10.3892/ijmm.2017.2969 [doi]
AB  - The present study aimed to explore the influence of aspirin on the CX3CL1/CX3CR1 
      signaling pathway in acute pulmonary embolism (APE) in rats. Our previous study 
      found that CX3CL1/CX3CR1 was increased in APE. However, the effect of this 
      signaling pathway on APE remains unclear. CX3CL1-shRNA adenovirus and 
      CX3CL1-overexpression vector were constructed. Male Sprague-Dawley rats were 
      randomly divided into 9 groups (n=10): normal group (group N), sham operation 
      group (group Sham), sham operation + aspirin group (group ASP), model group 
      (group M), model + ASP group (group M+A), model + shRNA group (group M+SH), sham 
      operation + CX3CL1-overexpression vector group (group Sham+Cx3), model + ASP + 
      shRNA group (group M+A+SH), and model + ASP + CX3CL1-overexpression vector group 
      (group M+A+CX3). Arterial pressure detection, hematoxylin and eosin staining, 
      reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent 
      assay, and laser confocal scanning microscopy were applied. Aspirin significantly 
      decreased pulmonary artery pressure, improve pathological changes in the 
      embolism, and decreased the expression of CX3CL1/CX3CR1 and CX3CL1/NF-kappaB. 
      Moreover, the adenovirus-overexpression CX3CL1 vector aggravated the inflammatory 
      changes in APE, which were improved by aspirin. However, the intervention of the 
      adenovirus CX3CL1 vector reduced the change, while its combination with aspirin 
      significantly improved the change. In conclusion, aspirin improved pathological 
      changes in rats with APE via the CX3CL1/CX3CR1 signaling pathway.
FAU - Zhang, Zhirong
AU  - Zhang Z
AD  - Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Yang, Weiji
AU  - Yang W
AD  - Postgraduates Department of Zhejiang Chinese Medical University, Hangzhou, 
      Zhejiang 310053, P.R. China.
FAU - Ying, Rongbiao
AU  - Ying R
AD  - Department of Surgical Oncology, Tumor Hospital of Taizhou, Wenling, Zhejiang 
      317500, P.R. China.
FAU - Shi, Ying
AU  - Shi Y
AD  - Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Jiang, Huifang
AU  - Jiang H
AD  - Department of Hematology, Tongde Hospital of Zhejiang, Hangzhou, Zhejiang 310012, 
      P.R. China.
FAU - Cai, Danli
AU  - Cai D
AD  - Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Kuang, Jing
AU  - Kuang J
AD  - Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, Zhejiang 310006, P.R. China.
FAU - Yang, Ruhui
AU  - Yang R
AD  - College of Medicine and Health, Lishui University, Lishui, Zhejiang 323000, P.R. 
      China.
FAU - Wang, Lingcong
AU  - Wang L
AD  - Department of ICU, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, Zhejiang 310006, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170426
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (CX3CR1 protein, rat)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Cx3cl1 protein, rat)
RN  - 0 (NF-kappa B)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
MH  - Aspirin/*therapeutic use
MH  - CX3C Chemokine Receptor 1/analysis/*immunology
MH  - Chemokine CX3CL1/analysis/*immunology
MH  - Male
MH  - NF-kappa B/analysis/immunology
MH  - Pulmonary Embolism/*drug therapy/immunology/pathology
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/*drug effects
EDAT- 2017/05/11 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/05/11 06:00
PHST- 2016/08/31 00:00 [received]
PHST- 2017/04/19 00:00 [accepted]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
PHST- 2017/05/11 06:00 [entrez]
AID - 10.3892/ijmm.2017.2969 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Jun;39(6):1580-1588. doi: 10.3892/ijmm.2017.2969. Epub 2017 
      Apr 26.