PMID- 28487966
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20180226
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Oligomeric proanthocyanidins protects A549 cells against H2O2-induced oxidative 
      stress via the Nrf2-ARE pathway.
PG  - 1548-1554
LID - 10.3892/ijmm.2017.2971 [doi]
AB  - Oxidative signaling and oxidative stress contribute to aging, cancer and diseases 
      resulting from lung fibrosis. In this study, we explored the anti-oxidative 
      potential of oligomeric proanthocyanidins (OPCs), natural flavonoid compounds. We 
      examined the protective effects of OPCs against hydrogen peroxide (H2O2)-induced 
      oxidative stress in non-small cell lung cancer cells (A549). We demonstrated that 
      OPC markedly attenuated H2O2-induced A549 cell viability, as shown by by 
      3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyl-tetrazolium bromide (MTT) assay. At 
      the same time, OPC inhibited H2O2-induced oxidative stress by significantly 
      increasing the activities of superoxide dismutase, catalase and glutathione, and 
      reducing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). 
      Treatment of the A549 cells with OPC significantly promoted the nuclear 
      translocation of NF-E2-related factor 2 (Nrf2) and significantly enhanced the 
      expression of its target genes [heme oxygenase-1 (HO-1), NAD(P)H quinone 
      dehydrogenase 1 (NQO1) and thioredoxin reductase 1 (TXNRD1)] with different fold 
      change values at both the mRNA and protein level. The knockout of Nrf2 using 
      CRISPR/Cas9 technology attenuated OPC-mediated ARE gene transcription, and almost 
      abolished the OPC-mediated protective effects against H2O2-induced oxidative 
      stress. On the whole, our study suggests that OPC plays an important role in 
      controlling the antioxidant response of A549 cells via the Nrf2-ARE pathway.
FAU - Sun, Chao
AU  - Sun C
AD  - Department of Cardiothoracic Surgery, Northern Jiangsu People's Hospital 
      Affiliated to Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
FAU - Jin, Weiguo
AU  - Jin W
AD  - Department of Cardiothoracic Surgery, Northern Jiangsu People's Hospital 
      Affiliated to Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
FAU - Shi, Hongcan
AU  - Shi H
AD  - Department of Cardiothoracic Surgery, Northern Jiangsu People's Hospital 
      Affiliated to Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170427
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Proanthocyanidins)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - A549 Cells
MH  - Antineoplastic Agents, Phytogenic/pharmacology
MH  - Antioxidant Response Elements/*drug effects
MH  - Antioxidants/*pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Lung Neoplasms/*drug therapy/genetics/metabolism
MH  - NF-E2-Related Factor 2/genetics/*metabolism
MH  - Oxidative Stress/drug effects
MH  - Proanthocyanidins/*pharmacology
MH  - Signal Transduction/*drug effects
EDAT- 2017/05/11 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/05/11 06:00
PHST- 2016/02/06 00:00 [received]
PHST- 2017/04/07 00:00 [accepted]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
PHST- 2017/05/11 06:00 [entrez]
AID - 10.3892/ijmm.2017.2971 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Jun;39(6):1548-1554. doi: 10.3892/ijmm.2017.2971. Epub 2017 
      Apr 27.