PMID- 28487970
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 15
IP  - 6
DP  - 2017 Jun
TI  - MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the 
      ERK/MAPK signaling pathway.
PG  - 3977-3982
LID - 10.3892/mmr.2017.6532 [doi]
AB  - Long non-coding RNA (lncRNA), transcripts of >200 bp in length that do not appear 
      to exhibit any coding capacity, are important in the occurrence and development 
      of cancer, cardiovascular and neurological diseases. However, effects of lncRNAs 
      on photo‑aging remain to be elucidated. To explore the potential effects of the 
      lncRNA metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1) on 
      photo‑aging in fibroblasts, MALAT1 expression was silenced in fibroblasts using 
      small interference RNA. Reverse transcription‑quantitative polymerase chain 
      reaction (RT‑qPCR) was used to examine MALAT1 expression in normal and silenced 
      fibroblasts following irradiation with 60 mJ/cm2 ultraviolet B (UVB) and an ELISA 
      assay was used to identify matrix metalloproteinase‑1 (MMP‑1) content in the 
      cellular supernatant. A beta-galactosidase kit was applied to measure the number of 
      senescent cells and a western blot assay was used to detect extracellular 
      signal‑regulated kinase (ERK), c‑Jun N‑terminal kinase (JNK) and p38 
      phosphorylation levels. RT‑qPCR was additionally used to detect changes in MALAT1 
      expression following suppression of UVB‑induced reactive oxygen species (ROS) 
      generation with N‑acetyl‑L‑cysteine (NAC). Fibroblasts irradiated with 60 mJ/cm2 
      UVB demonstrated increased MALAT1 expression, MMP‑1 secretory volume and number 
      of senescent cells, and greater levels of ERK, p38 and JNK phosphorylation. 
      Following silencing of MALAT1 expression in photo‑aged fibroblasts, decreases 
      were observed in MMP‑1 secretory volume, number of senescent cells and 
      phosphorylation levels of ERK. NAC reduced ROS content, however, it did not 
      affect MALAT1 expression. Therefore, it was concluded that MALAT1 may participate 
      in UVB‑induced photo‑aging via regulation of the ERK/mitogen‑activated protein 
      kinase signaling pathway and UVB‑induced MALAT1 expression is independent of ROS 
      generation.
FAU - Lei, Li
AU  - Lei L
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Zeng, Qinghai
AU  - Zeng Q
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Lu, Jianyun
AU  - Lu J
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Ding, Shu
AU  - Ding S
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Xia, Fang
AU  - Xia F
AD  - Department of Oncology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Kang, Jian
AU  - Kang J
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Tan, Lina
AU  - Tan L
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Gao, Lihua
AU  - Gao L
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Kang, Liyang
AU  - Kang L
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Cao, Ke
AU  - Cao K
AD  - Department of Oncology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Zhou, Jianda
AU  - Zhou J
AD  - Department of Plastic and Reconstructive Surgery, Third Xiangya Hospital of 
      Central South University, Changsha, Hunan 410013, P.R. China.
FAU - Xiao, Rong
AU  - Xiao R
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan 410011, P.R. China.
FAU - Chen, Jing
AU  - Chen J
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Huang, Jinhua
AU  - Huang J
AD  - Department of Dermatology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170428
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Biomarkers
MH  - Cell Survival
MH  - Cellular Senescence/*genetics/*radiation effects
MH  - Fibroblasts/metabolism/radiation effects
MH  - Gene Expression
MH  - Humans
MH  - MAP Kinase Signaling System/*radiation effects
MH  - Male
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Phosphorylation
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Small Interfering/genetics
MH  - Reactive Oxygen Species
MH  - Ultraviolet Rays/*adverse effects
PMC - PMC5436239
EDAT- 2017/05/11 06:00
MHDA- 2018/03/06 06:00
PMCR- 2017/04/28
CRDT- 2017/05/11 06:00
PHST- 2016/01/16 00:00 [received]
PHST- 2017/02/02 00:00 [accepted]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
PHST- 2017/05/11 06:00 [entrez]
PHST- 2017/04/28 00:00 [pmc-release]
AID - mmr-15-06-3977 [pii]
AID - 10.3892/mmr.2017.6532 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Jun;15(6):3977-3982. doi: 10.3892/mmr.2017.6532. Epub 2017 Apr 
      28.