PMID- 28493081
OWN - NLM
STAT- MEDLINE
DCOM- 20180330
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 40
IP  - 4
DP  - 2017 Aug
TI  - Regulation of iNOS-Derived ROS Generation by HSP90 and Cav-1 in Porcine 
      Reproductive and Respiratory Syndrome Virus-Infected Swine Lung Injury.
PG  - 1236-1244
LID - 10.1007/s10753-017-0566-9 [doi]
AB  - In the lungs, endothelial nitric oxide synthase (eNOS) is usually expressed in 
      endothelial cells and inducible nitric oxide synthase (iNOS) is mainly expressed 
      in alveolar macrophages and epithelial cells. Both eNOS and iNOS are involved in 
      lung inflammation. While they play several roles in lung inflammation formation 
      and resolution, their expression and activity are also regulated by inflammatory 
      factors. Their expression relationship in virus infection-induced lung injury is 
      not well addressed. In this report, we analyzed expression of both eNOS and iNOS, 
      the production of nitric oxide (NO) and reactive oxygen species (ROS), and 
      expression of their associated regulatory proteins, heat shock protein 90 (HSP90) 
      and caveolin-1 (Cav-1), in a swine lung injury model induced by porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. The combination of 
      upregulation of iNOS and downregulation of eNOS was observed in both natural and 
      experimental PRRSV-infected lungs, while the combination is much enhanced in 
      natural infected lungs. While NO production is much reduced in both infections, 
      ROS was enhanced only in natural infected lungs. Moreover, HSP90 is increased in 
      both natural and experimental infection and less Cav-1 expressed was observed 
      only in the natural PRRSV-infected lungs. Therefore, the increased ROS generation 
      is likely due to the increased iNOS and its unbalanced regulation by HSP90 and 
      Cav-1, and it also likely causes higher endothelial dysfunction in clinical 
      PRRSV-infected lungs.
FAU - Yan, Meiping
AU  - Yan M
AD  - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Hou, Make
AU  - Hou M
AD  - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Liu, Jie
AU  - Liu J
AD  - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Zhang, Songlin
AU  - Zhang S
AD  - Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, 256600, 
      Shandong Province, People's Republic of China.
FAU - Liu, Bang
AU  - Liu B
AD  - Department of Animal Genetics and Breeding, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei 
      Province, People's Republic of China. liubang@mail.hzau.edu.cn.
AD  - Key Lab of Swine Genetics and Breeding and Agricultural Animal Breeding and 
      Reproduction, College of Animal Science and Veterinary Medicine, Huazhong 
      Agricultural University, Wuhan, 430070, Hubei Province, People's Republic of 
      China. liubang@mail.hzau.edu.cn.
FAU - Wu, Xiaoxiong
AU  - Wu X
AD  - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Liu, Guoquan
AU  - Liu G
AD  - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, 
      People's Republic of China. liuguoquan@mail.hzau.edu.cn.
AD  - Key Lab of Swine Genetics and Breeding and Agricultural Animal Breeding and 
      Reproduction, College of Animal Science and Veterinary Medicine, Huazhong 
      Agricultural University, Wuhan, 430070, Hubei Province, People's Republic of 
      China. liuguoquan@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Caveolin 1)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Animals
MH  - Caveolin 1/metabolism/physiology
MH  - HSP90 Heat-Shock Proteins/metabolism/physiology
MH  - Lung Injury/enzymology/metabolism/*virology
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/*metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Swine
OTO - NOTNLM
OT  - caveolin-1
OT  - endothelial nitric oxide synthase
OT  - heat shock protein 90
OT  - inducible nitric oxide synthase
OT  - lung injury
OT  - reactive oxygen species
EDAT- 2017/05/12 06:00
MHDA- 2018/03/31 06:00
CRDT- 2017/05/12 06:00
PHST- 2017/05/12 06:00 [pubmed]
PHST- 2018/03/31 06:00 [medline]
PHST- 2017/05/12 06:00 [entrez]
AID - 10.1007/s10753-017-0566-9 [pii]
AID - 10.1007/s10753-017-0566-9 [doi]
PST - ppublish
SO  - Inflammation. 2017 Aug;40(4):1236-1244. doi: 10.1007/s10753-017-0566-9.