PMID- 28494372
OWN - NLM
STAT- MEDLINE
DCOM- 20180813
LR  - 20180813
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 184
DP  - 2017 Sep 5
TI  - Separation and preconcentration of riboflavin from human plasma using 
      polythionine coated magnetite/hydroxyapatite nanocomposite prior to analysis by 
      surfactant-enhanced fluorimetry.
PG  - 109-118
LID - S1386-1425(17)30365-7 [pii]
LID - 10.1016/j.saa.2017.04.087 [doi]
AB  - The exploration of novel adsorption properties of conductive polymers based on 
      hybridization with biocompatible nanomaterials receives an increasing interest. 
      In this regard, hydroxyapatite (HA) bioceramic is of critical importance mainly 
      owing to its facile synthesis, high surface area, economic and low toxicity in 
      biological environments. In this work, we first prepared and characterized a 
      magnetite/hydroxyapatite (Fe(3)O(4)/HA) nanocomposite using the bio-waste chicken 
      eggshell via an attractive green way that involved low cost and irrespective of 
      toxicity. Then, polythionine as a novel class of conductive polymers was in situ 
      coated on the synthesized magnetic bioceramic for the separation and 
      preconcentration of riboflavin (vitamin B(2)) in human plasma before its 
      fluorimetric determination. Considering the putative role of riboflavin in 
      protecting against cancer and cardiovascular diseases, it is essential to 
      evaluate this vitamin in biological fluids. The described method possesses a 
      linear range of 0.75-262.5mugL(-1) (R(2)=0.9985) and a detection limit of 
      0.20mugL(-1) (signal-to-noise ratio of 3). The relative standard deviations (RSDs) 
      for single-sorbent repeatability and sorbent-to-sorbent reproducibility were less 
      than 4.0% and 7.6% (n=5), respectively. The respective enrichment factor and 
      extraction recovery of the method found to be 35.7 and 98.4%. The analytical 
      performance of method for riboflavin was characterized by good consistency of the 
      results with those obtained by the enzyme-linked immunosorbent assay (ELISA) 
      conventional method (p-value of <0.05). The optimized protocol intended for 
      control determinations of riboflavin in human subjects and is addressed to 
      clinical laboratories.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Farzin, Leila
AU  - Farzin L
AD  - Radiation Application Research School, Nuclear Science and Technology Research 
      Institute, P.O. Box 11365-3486, Tehran, Iran. Electronic address: 
      lfarzin84@yahoo.com.
FAU - Shamsipur, Mojtaba
AU  - Shamsipur M
AD  - Department of Chemistry, Razi University, P.O. Box 67149-67346, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
DEP - 20170502
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Phenothiazines)
RN  - 0 (Polymers)
RN  - 0 (Surface-Active Agents)
RN  - 91D9GV0Z28 (Durapatite)
RN  - TLM2976OFR (Riboflavin)
RN  - VTT2SAT5H0 (thionine)
SB  - IM
MH  - Adult
MH  - Durapatite/*chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Magnetite Nanoparticles/*chemistry
MH  - Nanocomposites/chemistry
MH  - Phenothiazines/chemistry
MH  - Polymers/chemistry
MH  - Reproducibility of Results
MH  - Riboflavin/*blood/*isolation & purification
MH  - Spectrometry, Fluorescence/*methods
MH  - Surface-Active Agents/*chemistry
OTO - NOTNLM
OT  - Fluorimetric determination
OT  - Hydroxyapatite
OT  - Polythionine
OT  - Riboflavin
EDAT- 2017/05/12 06:00
MHDA- 2018/08/14 06:00
CRDT- 2017/05/12 06:00
PHST- 2017/01/18 00:00 [received]
PHST- 2017/04/19 00:00 [revised]
PHST- 2017/04/30 00:00 [accepted]
PHST- 2017/05/12 06:00 [pubmed]
PHST- 2018/08/14 06:00 [medline]
PHST- 2017/05/12 06:00 [entrez]
AID - S1386-1425(17)30365-7 [pii]
AID - 10.1016/j.saa.2017.04.087 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2017 Sep 5;184:109-118. doi: 
      10.1016/j.saa.2017.04.087. Epub 2017 May 2.