PMID- 28496138
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 May 11
TI  - Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes 
      in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical 
      correlations.
PG  - 1725
LID - 10.1038/s41598-017-01895-x [doi]
LID - 1725
AB  - The aim of this study was to investigate the hyperacute and acute changes in 
      apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after 
      severe bilateral renal ischemic-reperfusion injury (IRI). After baseline MRI, 24 
      Spraque-Dawley rats with renal IRI were divided equally as group 1 (post-IRI MRI 
      at 6 hours, days 1, 3, and 7) and groups 2, 3, and 4 (post-IRI MRI at 6 hours; 
      6 hours and day 1; 6 hours, days 1 and 3, respectively), while six other rats 
      without IRI (group 5) were used as sham control. ADC, T1, and T2 values of the 
      cortex and outer and inner stripes of outer medulla (OSOM and ISOM), and 
      immunohistochemical studies assessing monocyte chemoattractant protein-1 (MCP-1), 
      CD68+ cells, tubular cast formation, and collagen deposition in three zones at 
      different time points were evaluated. Significantly reduced ADCs in OSOM and ISOM 
      are noninvasive biomarkers denoting hyperacute damages after IRI. Linear 
      regression analysis revealed a significant inverse correlation between 
      6-hour/baseline ADC ratios and MCP-1 staining (P < 0.001, r(2) = 0.738). ADC, T1, 
      and T2 values are useful for assessing variable IRI changes in different layers 
      depending on underlying microstructural and histopathological changes at 
      different time points.
FAU - Ko, Sheung-Fat
AU  - Ko SF
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan. sfatko@adm.cgmh.org.tw.
FAU - Yip, Hon-Kan
AU  - Yip HK
AUID- ORCID: 0000-0002-6305-5717
AD  - Department of Cardiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Zhen, Yen-Yi
AU  - Zhen YY
AD  - Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Lee, Chia-Chang
AU  - Lee CC
AD  - Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Lee, Chen-Chang
AU  - Lee CC
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
AD  - Department of Biomedical Engineering, I-Shou University, Kaohsiung, 833, Taiwan.
FAU - Huang, Shyh-Jer
AU  - Huang SJ
AD  - Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Huang, Chung-Cheng
AU  - Huang CC
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Ng, Shu-Hang
AU  - Ng SH
AD  - Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
FAU - Lin, Jui-Wei
AU  - Lin JW
AD  - Department of Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, 833, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170511
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Animals
MH  - Creatinine/blood
MH  - Diffusion
MH  - Immunohistochemistry
MH  - Kidney/*diagnostic imaging/*injuries/pathology
MH  - Kidney Cortex/diagnostic imaging/pathology
MH  - *Magnetic Resonance Imaging
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Regression Analysis
MH  - Reperfusion Injury/blood/*pathology
MH  - Time Factors
PMC - PMC5431885
COIS- The authors declare that they have no competing interests.
EDAT- 2017/05/13 06:00
MHDA- 2018/12/12 06:00
PMCR- 2017/05/11
CRDT- 2017/05/13 06:00
PHST- 2016/12/05 00:00 [received]
PHST- 2017/04/04 00:00 [accepted]
PHST- 2017/05/13 06:00 [entrez]
PHST- 2017/05/13 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/05/11 00:00 [pmc-release]
AID - 10.1038/s41598-017-01895-x [pii]
AID - 1895 [pii]
AID - 10.1038/s41598-017-01895-x [doi]
PST - epublish
SO  - Sci Rep. 2017 May 11;7(1):1725. doi: 10.1038/s41598-017-01895-x.