PMID- 28499834
OWN - NLM
STAT- MEDLINE
DCOM- 20180525
LR  - 20180525
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 123
DP  - 2017 Oct
TI  - N-stearoyltyrosine protects primary cortical neurons against oxygen-glucose 
      deprivation-induced apoptosis through inhibiting anandamide inactivation system.
PG  - 8-18
LID - S0168-0102(17)30012-3 [pii]
LID - 10.1016/j.neures.2017.04.019 [doi]
AB  - N-stearoylthrosine (NST), a synthesized anandamide (AEA) analogue, plays a 
      neuroprotective role in neurodegenerative diseases and cerebrovascular diseases. 
      Several studies have demonstrated that the endocannabinoids systems (ECS) are 
      involved in the neuroprotective effects against cerebral ischemic injury. 
      Oxygen-glucose deprivation (OGD)-induced neuronal injury elevated the levels of 
      endocannabinoids and activated ECS. This research was conducted to investigate 
      the neuroprotective effect of NST against OGD-induced neuronal injury in cultured 
      primary cortical neurons and the potential mechanism involved. Cortical neurons 
      were treated with NST at indicate concentrations for 30min prior to injury and 
      OGD injured neurons were incubated with normal conditions for 0-24h. The best 
      neuroprotective effect of NST against OGD-induced injury occurred at 10muM. All 
      data indicated that the neuroprotective effect of NST against OGD-induced injury 
      resulted from blocking anandamide membrane transporter (AMT) (IC(50)=11.74nM) and 
      inhibiting fatty acid amide hydrolase activity (FAAH) (IC(50)=16.54nM). Our 
      findings demonstrated that NST has an important role in cerebral ischemic injury 
      pathological progression through activating cannabinoid receptors by inhibiting 
      AEA inactivation system. These data suggested a potential role for NST in the 
      therapeutic consideration of cerebral ischemic injury. However, inhibition of AEA 
      inactivation system may provide a neuroprotective effect during cerebral ischemic 
      injury.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Cui, Heng-Jing
AU  - Cui HJ
AD  - Department of Pharmacy, RuiJin Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai 200025, PR China.
FAU - Liu, Sha
AU  - Liu S
AD  - Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, 
      Shanghai 200025, PR China.
FAU - Yang, Rui
AU  - Yang R
AD  - Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, 
      Shanghai 200025, PR China.
FAU - Fu, Guo-Hui
AU  - Fu GH
AD  - Department of Pathology, Shanghai Jiao Tong University School of Medicine, 
      Shanghai 200025, PR China.
FAU - Lu, Yang
AU  - Lu Y
AD  - Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, 
      Shanghai 200025, PR China. Electronic address: huaxue@shsmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170510
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Arachidonic Acids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 42HK56048U (Tyrosine)
RN  - 57993-25-6 (N-stearoyltyrosine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.- (fatty-acid amide hydrolase)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Amidohydrolases/metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Arachidonic Acids/*metabolism
MH  - Cell Hypoxia/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Embryo, Mammalian
MH  - Endocannabinoids/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/*deficiency/pharmacology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Neurons/*drug effects
MH  - Nitric Oxide/metabolism
MH  - Oxygen/pharmacology
MH  - Polyunsaturated Alkamides/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tyrosine/*analogs & derivatives/pharmacology
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Anandamide (AEA)
OT  - Anandamide inactivation system
OT  - Endocannabinoid system (ECS)
OT  - N-stearoylthrosine (NST)
OT  - Oxygen-glucose deprivation (OGD)
EDAT- 2017/05/14 06:00
MHDA- 2018/05/26 06:00
CRDT- 2017/05/14 06:00
PHST- 2017/01/06 00:00 [received]
PHST- 2017/04/13 00:00 [revised]
PHST- 2017/04/17 00:00 [accepted]
PHST- 2017/05/14 06:00 [pubmed]
PHST- 2018/05/26 06:00 [medline]
PHST- 2017/05/14 06:00 [entrez]
AID - S0168-0102(17)30012-3 [pii]
AID - 10.1016/j.neures.2017.04.019 [doi]
PST - ppublish
SO  - Neurosci Res. 2017 Oct;123:8-18. doi: 10.1016/j.neures.2017.04.019. Epub 2017 May 
      10.