PMID- 28499858
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20201209
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 74
DP  - 2017 Sep
TI  - Enhanced behavioral immune defenses in a C. elegans C-type lectin-like domain 
      gene mutant.
PG  - 237-242
LID - S0145-305X(17)30141-6 [pii]
LID - 10.1016/j.dci.2017.04.021 [doi]
AB  - C-type lectin-like domain (CTLD) proteins occupy crucial functions in the immune 
      system of vertebrates, but their role in invertebrate immunity is much less 
      understood. The nematode Caenorhabditis elegans possesses a highly diverse CTLD 
      protein encoding (clec) gene repertoire. A role of C. elegans clec genes in 
      pathogen defense is always assumed, yet experimental evidence for clec immune 
      function is rare. To systematically test the potential function of clec genes in 
      the C. elegans defense against pathogens, we screened 39 clec mutants for 
      survival on the Gram-positive pathogen Bacillus thuringiensis (BT18247) and 37 
      clec mutants on the Gram-negative pathogen Pseudomonas aeruginosa (PA14). We 
      found that clec mutants can exhibit either decreased or, unexpectedly, increased 
      resistance to infection. Since we observed high escape behavior for some of the 
      clec mutants on BT18247 during the initial screen, we then asked if increased 
      pathogen avoidance behavior underlies the increased resistance of some clec 
      mutants. We thus tested lawn leaving behavior of the resistant clec-29(ok3181), 
      clec-34(ok2120), clec-151(ok2264), and C54G4.4(ok2110) mutant on BT18247. We 
      found that C54G4.4(ok2110) mutant animals exhibit a particularly strong lawn 
      leaving behavior, in addition to prolonged feeding cessation when exposed to 
      BT18247. Together, our results indicate that clec genes mediate both resistance 
      and susceptibility to infection. Further, behavioral analyses of the 
      C54G4.4(ok2110) mutant implicate C54G4.4 in the regulation of pathogen avoidance 
      behavior towards BT18247. We conclude that C. elegans clec genes may act both as 
      positive and negative regulators of physiological as well as behavioral immune 
      defense responses.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Pees, Barbara
AU  - Pees B
AD  - Department of Evolutionary Ecology and Genetics, Christian-Albrechts-Universitat 
      zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
FAU - Kloock, Anke
AU  - Kloock A
AD  - Department of Evolutionary Ecology and Genetics, Christian-Albrechts-Universitat 
      zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
FAU - Nakad, Rania
AU  - Nakad R
AD  - Department of Evolutionary Ecology and Genetics, Christian-Albrechts-Universitat 
      zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
FAU - Barbosa, Camilo
AU  - Barbosa C
AD  - Department of Evolutionary Ecology and Genetics, Christian-Albrechts-Universitat 
      zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
FAU - Dierking, Katja
AU  - Dierking K
AD  - Department of Evolutionary Ecology and Genetics, Christian-Albrechts-Universitat 
      zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany. Electronic address: 
      kdierking@zoologie.uni-kiel.de.
LA  - eng
PT  - Journal Article
DEP - 20170510
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Lectins, C-Type)
SB  - IM
MH  - Animals
MH  - Bacillus thuringiensis/*immunology
MH  - Bacterial Infections/*immunology
MH  - *Behavior, Animal
MH  - Caenorhabditis elegans/*immunology
MH  - Caenorhabditis elegans Proteins/genetics/*metabolism
MH  - Immunity, Innate
MH  - Lectins, C-Type/*genetics/metabolism
MH  - Mutation/*genetics
MH  - Nematode Infections/*immunology
MH  - Protein Domains/*genetics
MH  - Pseudomonas aeruginosa/*immunology
OTO - NOTNLM
OT  - Avoidance behavior
OT  - Bacillus thuringiensis
OT  - C-type lectins
OT  - C. elegans
OT  - Immune defenses
OT  - Resistance
EDAT- 2017/05/14 06:00
MHDA- 2018/03/10 06:00
CRDT- 2017/05/14 06:00
PHST- 2017/03/17 00:00 [received]
PHST- 2017/04/19 00:00 [revised]
PHST- 2017/04/19 00:00 [accepted]
PHST- 2017/05/14 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
PHST- 2017/05/14 06:00 [entrez]
AID - S0145-305X(17)30141-6 [pii]
AID - 10.1016/j.dci.2017.04.021 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2017 Sep;74:237-242. doi: 10.1016/j.dci.2017.04.021. Epub 2017 
      May 10.