PMID- 28499977
OWN - NLM
STAT- MEDLINE
DCOM- 20180328
LR  - 20180919
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 355
DP  - 2017 Jul 4
TI  - Human neural stem/progenitor cells derived from the olfactory epithelium express 
      the TrkB receptor and migrate in response to BDNF.
PG  - 84-100
LID - S0306-4522(17)30315-9 [pii]
LID - 10.1016/j.neuroscience.2017.04.047 [doi]
AB  - Neurogenesis constitutively occurs in the olfactory epithelium of mammals, 
      including humans. The fact that new neurons in the adult olfactory epithelium 
      derive from resident neural stem/progenitor cells suggests a potential use for 
      these cells in studies of neural diseases, as well as in neuronal cell 
      replacement therapies. In this regard, some studies have proposed that the human 
      olfactory epithelium is a source of neural stem/progenitor cells for autologous 
      transplantation. Although these potential applications are interesting, it is 
      important to understand the cell biology and/or whether human neural 
      stem/progenitor cells in the olfactory epithelium sense external signals, such as 
      brain-derived neurotrophic factor (BDNF), that is also found in other 
      pro-neurogenic microenvironments. BDNF plays a key role in several biological 
      processes, including cell migration. Thus, we characterized human neural 
      stem/progenitor cells derived from the olfactory epithelium (hNS/PCs-OE) and 
      studied their in vitro migratory response to BDNF. In the present study, we 
      determined that hNS/PCs-OE express the protein markers Nestin, Sox2, Ki67 and 
      betaIII-tubulin. Moreover, the doubling time of hNS/PCs-OE was approximately 38h. 
      Additionally, we found that hNS/PCs-OE express the BDNF receptor TrkB, and 
      pharmacological approaches showed that the BDNF-induced (40ng/ml) migration of 
      differentiated hNS/PCs-OE was affected by the compound K252a, which prevents TrkB 
      activation. This observation was accompanied by changes in the number of vinculin 
      adhesion contacts. Our results suggest that hNS/PCs-OE exhibit a migratory 
      response to BDNF, accompanied by the turnover of adhesion contacts.
CI  - Copyright (c) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Ortiz-Lopez, Leonardo
AU  - Ortiz-Lopez L
AD  - Laboratory of Neurogenesis, Division of Clinical Investigations, National 
      Institute of Psychiatry "Ramon de la Fuente Muniz", Calzada Mexico-Xochimilco 
      101, 14370 Ciudad de Mexico, Mexico.
FAU - Gonzalez-Olvera, Jorge Julio
AU  - Gonzalez-Olvera JJ
AD  - Division of Clinical Investigations, National Institute of Psychiatry "Ramon de 
      la Fuente Muniz", Calzada Mexico-Xochimilco 101, 14370 Ciudad de Mexico, Mexico.
FAU - Vega-Rivera, Nelly Maritza
AU  - Vega-Rivera NM
AD  - Laboratory of Neuropsychopharmacology, Division of Neuroscience, National 
      Institute of Psychiatry "Ramon de la Fuente Muniz", Calzada Mexico-Xochimilco 
      101, 14370 Ciudad de Mexico, Mexico.
FAU - Garcia-Anaya, Maria
AU  - Garcia-Anaya M
AD  - Division of Clinical Investigations, National Institute of Psychiatry "Ramon de 
      la Fuente Muniz", Calzada Mexico-Xochimilco 101, 14370 Ciudad de Mexico, Mexico.
FAU - Carapia-Hernandez, Ana Karen
AU  - Carapia-Hernandez AK
AD  - Laboratory of Neurogenesis, Division of Clinical Investigations, National 
      Institute of Psychiatry "Ramon de la Fuente Muniz", Calzada Mexico-Xochimilco 
      101, 14370 Ciudad de Mexico, Mexico.
FAU - Velazquez-Escobar, Julio Cesar
AU  - Velazquez-Escobar JC
AD  - Laboratory of Neurogenesis, Division of Clinical Investigations, National 
      Institute of Psychiatry "Ramon de la Fuente Muniz", Calzada Mexico-Xochimilco 
      101, 14370 Ciudad de Mexico, Mexico.
FAU - Ramirez-Rodriguez, Gerardo Bernabe
AU  - Ramirez-Rodriguez GB
AD  - Laboratory of Neurogenesis, Division of Clinical Investigations, National 
      Institute of Psychiatry "Ramon de la Fuente Muniz", Calzada Mexico-Xochimilco 
      101, 14370 Ciudad de Mexico, Mexico. Electronic address: gbernabe@imp.edu.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170510
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Histones)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tubulin)
RN  - 125361-02-6 (Vinculin)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Carbazoles/pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Colchicine/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Histones/metabolism
MH  - Humans
MH  - Indole Alkaloids/pharmacology
MH  - Ki-67 Antigen/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neural Stem Cells/*drug effects
MH  - Olfactory Mucosa/*cytology
MH  - Receptor, trkB/*metabolism
MH  - Time Factors
MH  - Tubulin/metabolism
MH  - Vinculin/metabolism
OTO - NOTNLM
OT  - BDNF
OT  - adult neurogenesis
OT  - migration
OT  - neural stem/progenitor cells
OT  - olfactory epithelium
OT  - vinculin
EDAT- 2017/05/14 06:00
MHDA- 2018/03/29 06:00
CRDT- 2017/05/14 06:00
PHST- 2017/03/11 00:00 [received]
PHST- 2017/04/27 00:00 [revised]
PHST- 2017/04/29 00:00 [accepted]
PHST- 2017/05/14 06:00 [pubmed]
PHST- 2018/03/29 06:00 [medline]
PHST- 2017/05/14 06:00 [entrez]
AID - S0306-4522(17)30315-9 [pii]
AID - 10.1016/j.neuroscience.2017.04.047 [doi]
PST - ppublish
SO  - Neuroscience. 2017 Jul 4;355:84-100. doi: 10.1016/j.neuroscience.2017.04.047. 
      Epub 2017 May 10.