PMID- 28501485
OWN - NLM
STAT- MEDLINE
DCOM- 20171127
LR  - 20180126
IS  - 1618-131X (Electronic)
IS  - 1438-4639 (Linking)
VI  - 220
IP  - 4
DP  - 2017 Jun
TI  - Rapid genotyping of Legionella pneumophila serogroup 1 strains by a novel DNA 
      microarray-based assay during the outbreak investigation in Warstein, Germany 
      2013.
PG  - 673-678
LID - S1438-4639(16)30006-2 [pii]
LID - 10.1016/j.ijheh.2016.02.004 [doi]
AB  - Between 1 August and 6 September 2013, an outbreak of Legionnaires' disease (LD) 
      with 78 cases confirmed by positive urinary antigen tests occurred in Warstein, 
      North Rhine-Westphalia, Germany. Legionella (L.) pneumophila, serogroup (Sg) 1, 
      monoclonal antibody (mAb) subgroup Knoxville, sequence type (ST) 345, was 
      identified as the epidemic strain. This strain was isolated from seven patients. 
      To detect the source of the infection, epidemiological typing of clinical and 
      environmental strains was performed in two consecutive steps. First, strains were 
      typed by monoclonal antibodies. Indistinguishable strains were further subtyped 
      by sequence-based typing (SBT) which is the internationally recognized standard 
      method for epidemiological genotyping of L. pneumophila. In an early stage of the 
      outbreak investigation, many environmental isolates were found to belong to the 
      mAb subgroup Knoxville, but to two different STs, namely to ST 345, the epidemic 
      strain, and to ST 600. A majority of environmental isolates belonged to ST 600 
      whereas the epidemic ST 345 strain was less common in environmental samples. To 
      rapidly distinguish both Knoxville strains, we applied a novel typing method 
      based on DNA-hybridization on glass chips. The new assay can easily and rapidly 
      discriminate L. pneumophila Sg 1 strains. Thus, we were able to quickly identify 
      the sources harboring the epidemic strain, i.e., two cooling towers of different 
      companies, the waste water treatment plants (WWTP) of the city and one company as 
      well as water samples of the river Wester and its branches.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Petzold, Markus
AU  - Petzold M
AD  - Institute of Medical Microbiology and Hygiene, German Reference Laboratory for 
      Legionella, Dresden University of Technology, Fetscherstrasse 74, 01307 Dresden, 
      Germany. Electronic address: markus.petzold@tu-dresden.de.
FAU - Ehricht, Ralf
AU  - Ehricht R
AD  - Alere Technologies GmbH, Lobstedter Strasse 103-105, 07749 Jena, Germany. 
      Electronic address: ralf@clondiag.com.
FAU - Slickers, Peter
AU  - Slickers P
AD  - Alere Technologies GmbH, Lobstedter Strasse 103-105, 07749 Jena, Germany. 
      Electronic address: piet@clondiag.com.
FAU - Pleischl, Stefan
AU  - Pleischl S
AD  - Institute for Hygiene and Public Health, University of Bonn, Sigmund-Freud-Strasse 
      25, 53105 Bonn, Germany. Electronic address: stefan.pleischl@ukb.uni-bonn.de.
FAU - Brockmann, Ansgar
AU  - Brockmann A
AD  - Regional Public Health Department Soest, Mastholter Strasse 230, 59558 Lippstadt, 
      Germany. Electronic address: ansgar.brockmann@kreis-soest.de.
FAU - Exner, Martin
AU  - Exner M
AD  - Institute for Hygiene and Public Health, University of Bonn, Sigmund-Freud-Strasse 
      25, 53105 Bonn, Germany. Electronic address: martin.exner@ukb.uni-bonn.de.
FAU - Monecke, Stefan
AU  - Monecke S
AD  - Institute of Medical Microbiology and Hygiene, German Reference Laboratory for 
      Legionella, Dresden University of Technology, Fetscherstrasse 74, 01307 Dresden, 
      Germany; Alere Technologies GmbH, Lobstedter Strasse 103-105, 07749 Jena, Germany. 
      Electronic address: stefan.monecke@clondiag.com.
FAU - Luck, Christian
AU  - Luck C
AD  - Institute of Medical Microbiology and Hygiene, German Reference Laboratory for 
      Legionella, Dresden University of Technology, Fetscherstrasse 74, 01307 Dresden, 
      Germany. Electronic address: christian.lueck@tu-dresden.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160222
PL  - Germany
TA  - Int J Hyg Environ Health
JT  - International journal of hygiene and environmental health
JID - 100898843
SB  - IM
MH  - Disease Outbreaks
MH  - Genotype
MH  - Germany/epidemiology
MH  - Humans
MH  - Legionella pneumophila/*genetics/isolation & purification
MH  - Legionnaires' Disease/epidemiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Serogroup
OTO - NOTNLM
OT  - Cooling tower
OT  - DNA-microarray typing
OT  - Legionella pneumophila serogroup 1
OT  - Legionnaires' disease
OT  - Molecular epidemiology
OT  - Outbreak
EDAT- 2016/02/22 00:00
MHDA- 2017/11/29 06:00
CRDT- 2017/05/15 06:00
PHST- 2015/12/11 00:00 [received]
PHST- 2016/02/12 00:00 [revised]
PHST- 2016/02/12 00:00 [accepted]
PHST- 2016/02/22 00:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/05/15 06:00 [entrez]
AID - S1438-4639(16)30006-2 [pii]
AID - 10.1016/j.ijheh.2016.02.004 [doi]
PST - ppublish
SO  - Int J Hyg Environ Health. 2017 Jun;220(4):673-678. doi: 
      10.1016/j.ijheh.2016.02.004. Epub 2016 Feb 22.