PMID- 28501766
OWN - NLM
STAT- MEDLINE
DCOM- 20180312
LR  - 20180312
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 48
DP  - 2017 Jul
TI  - Therapeutic effects of zerumbone in an alkali-burned corneal wound healing model.
PG  - 126-134
LID - S1567-5769(17)30176-5 [pii]
LID - 10.1016/j.intimp.2017.05.005 [doi]
AB  - Cornea is an avascular transparent tissue. Ocular trauma caused by a corneal 
      alkali burn induces corneal neovascularization (CNV), inflammation, and fibrosis, 
      leading to vision loss. The purpose of this study was to examine the effects of 
      Zerumbone (ZER) on corneal wound healing caused by alkali burns in mice. CNV was 
      induced by alkali-burn injury in BALB/C female mice. Topical ZER (three times per 
      day, 3mul each time, at concentrations of 5, 15, and 30muM) was applied to treat 
      alkali-burned mouse corneas for 14 consecutive days. Histopathologically, ZER 
      treatment suppressed alkali burn-induced CNV and decreased corneal epithelial 
      defects induced by alkali burns. Corneal tissue treated with ZER showed reduced 
      mRNA levels of pro-angiogenic genes, including vascular endothelial growth 
      factor, matrix metalloproteinase-2 and 9, and pro-fibrotic factors such as alpha 
      smooth muscle actin and transforming growth factor-1 and 2. Immunohistochemical 
      analysis demonstrated that the infiltration of F4/80 and/or CCR2 positive cells 
      was significantly decreased in ZER-treated corneas. ZER markedly inhibited the 
      mRNA and protein levels of monocyte chemoattractant protein-1 (MCP-1) in human 
      corneal fibroblasts and murine peritoneal macrophages. Immunoblot analysis 
      revealed that ZER decreased the activation of signal transducer and activator of 
      transcription 3 (STAT3), with consequent reduction of MCP-1 production by these 
      cells. In conclusion, topical administration of ZER accelerated corneal wound 
      healing by inhibition of STAT3 and MCP-1 production.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Kim, Jong Won
AU  - Kim JW
AD  - Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), 
      College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea.
FAU - Jeong, Hyuneui
AU  - Jeong H
AD  - Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), 
      College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea.
FAU - Yang, Myeon-Sik
AU  - Yang MS
AD  - Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), 
      College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea.
FAU - Lim, Chae Woong
AU  - Lim CW
AD  - Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), 
      College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea.
FAU - Kim, Bumseok
AU  - Kim B
AD  - Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), 
      College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea. Electronic address: bskims@jbnu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Alkalies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Sesquiterpenes)
RN  - 471-05-6 (zerumbone)
SB  - IM
MH  - Alkalies
MH  - Animals
MH  - Burns, Chemical/*drug therapy/metabolism/pathology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/antagonists & inhibitors/genetics/metabolism
MH  - Cornea/drug effects/metabolism/pathology
MH  - Corneal Injuries/chemically induced/*drug therapy/metabolism/pathology
MH  - Corneal Neovascularization/chemically induced/*drug therapy/metabolism/pathology
MH  - Eye Burns/chemically induced/*drug therapy/metabolism/pathology
MH  - Female
MH  - Fibroblasts/drug effects/metabolism
MH  - Humans
MH  - Macrophages, Peritoneal/drug effects/metabolism
MH  - Mice, Inbred BALB C
MH  - STAT3 Transcription Factor/antagonists & inhibitors/metabolism
MH  - Sesquiterpenes/pharmacology/*therapeutic use
MH  - Wound Healing/drug effects
OTO - NOTNLM
OT  - Alkali-burned injury
OT  - CCL2/MCP-1
OT  - Cornea
OT  - STAT3
OT  - Zerumbone
EDAT- 2017/05/16 06:00
MHDA- 2018/03/13 06:00
CRDT- 2017/05/15 06:00
PHST- 2017/01/17 00:00 [received]
PHST- 2017/04/17 00:00 [revised]
PHST- 2017/05/04 00:00 [accepted]
PHST- 2017/05/16 06:00 [pubmed]
PHST- 2018/03/13 06:00 [medline]
PHST- 2017/05/15 06:00 [entrez]
AID - S1567-5769(17)30176-5 [pii]
AID - 10.1016/j.intimp.2017.05.005 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2017 Jul;48:126-134. doi: 10.1016/j.intimp.2017.05.005. Epub 
      2017 May 11.