PMID- 28502130
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20181202
IS  - 1110-4902 (Print)
VI  - 23
IP  - 2
DP  - 2016 Jun
TI  - Diagnostic and Prognostic Values of Monocyte Chemotactic Protein-1 in Ascitic 
      Fluid of Patients with Spontaneous Bacterial Peritonitis.
PG  - 17-27
AB  - Spontaneous bacterial peritonitis (SBP) is a severe complication in cirrhotics 
      with ascites. Monocyte chemotactic protien-1 (MCP-1) is a chemotactic factor for 
      monocytes/macrophages, and it activates lymphocytes and neutrophils during 
      infection. This study aimed to evaluate the role of MPC-1 in the pathogenesis of 
      SBP and assess its prognostic value and correlation to disease severity. The 
      study included ninety patients with liver cirrhosis and ascites. Patients were 
      divided into 2 groups: Group I including 45 ascetic patients with SBP (polymorph 
      nuclear cell count (PMN) >= 250 cell/mm3 in ascitic fluid), and Group II 
      including 45 ascetic patients without SBP. Assessment of the severity of liver 
      cirrhosis was done using the modified Child-Pugh and model for end stage liver 
      disease (MELD) scores. Ascetic fluid samples were subjected to total leucocytic 
      count and differential, albumin, protein, glucose, and serum-ascetic albumin 
      gradient analysis Ascetic fluid levels of (MCP-1was measured by ELISA. Higher 
      level was detected in patients with SBP as compared to those without SBP. The 
      number of polymorph nuclear cell count (PMN) >= 250 cell/mm3 in ascitic fluid) 
      was used as gold standard for diagnosis of SBP. The diagnosis sensitivity and 
      specificity of MCP level test were 86.7% and 95.4% respectively at cutoff 
      of122.5ng/ml with accuracy 91%. MCP-1 level showed positive significant 
      correlation with TLC, PMN leucocytes and MELD score. In conclusion, ascitic fluid 
      MCP-1 level could be a reliable test for diagnosis of SBP, and could be used as a 
      prognostic marker due to its positive correlation with the severity of liver 
      disease.
CI  - Copyright(c) by the Egyptian Association of Immunologists.
FAU - El-Toukhy, Naglaa
AU  - El-Toukhy N
AD  - Department of Hepatology & Gastroenterology & Infectious diseases,Faculty of 
      Medicine, Benha University, Benha, Egypt.
FAU - Emam, Sherin M
AU  - Emam SM
AD  - Department of Medical Microbiology & Immunology,Faculty of Medicine, Benha 
      University, Benha, Egypt.
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Ascitic Fluid/chemistry
MH  - Bacterial Infections/*diagnosis
MH  - Chemokine CCL2/*analysis
MH  - Humans
MH  - Liver Cirrhosis/complications
MH  - Peritonitis/*diagnosis
MH  - Prognosis
EDAT- 2017/05/16 06:00
MHDA- 2018/07/24 06:00
CRDT- 2017/05/15 06:00
PHST- 2017/05/15 06:00 [entrez]
PHST- 2017/05/16 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PST - ppublish
SO  - Egypt J Immunol. 2016 Jun;23(2):17-27.