PMID- 28505030
OWN - NLM
STAT- MEDLINE
DCOM- 20190619
LR  - 20210115
IS  - 1528-1159 (Electronic)
IS  - 0362-2436 (Linking)
VI  - 43
IP  - 1
DP  - 2018 Jan 1
TI  - Association Between Neurotrophic Factor Expression and Pain-Related Behavior 
      Induced by Nucleus Pulposus Applied to Rat Nerve Root.
PG  - E7-E15
LID - 10.1097/BRS.0000000000002223 [doi]
AB  - STUDY DESIGN: An experimental animal study. OBJECTIVE: To investigate the 
      relationship between pain-related behavior and the expression of neurotrophic 
      factors in the dorsal root ganglion (DRG) and spinal cord (SC) using a nucleus 
      pulposus (NP) rat model. SUMMARY OF BACKGROUND DATA: Neurotrophic factors are 
      released from activated glial cells and are associated with pain-related 
      behavior. Nerve growth factor (NGF) is a neurotrophic factor that is induced by 
      inflammation. METHODS: Rats were divided into an NP group (n = 94) and a 
      sham-operated group (n = 46). NP harvested from the tail was applied to the left 
      L5 DRG. Rats in the NP group were then divided into five subgroups: one 
      non-treatment and four treatment groups. In the treatment groups, a dose of 
      anti-NGF antibody or phosphate-buffered saline was administered into the DRG. 
      Behavioral testing was performed to investigate the mechanical withdrawal 
      threshold of the left hind paw for all groups. Immunohistochemical localization 
      of NGF, phosphorylated p38 (p38), and brain-derived neurotrophic factor (BDNF) in 
      the DRGs and SCs was performed, and the numbers of immunoreactive (IR) cells were 
      counted. RESULTS: The withdrawal threshold in the nontreatment NP group was 
      significantly decreased for 35 days, and that of the middle- and high-dose 
      treatment rats was significantly higher than the phosphate-buffered saline group 
      values. In the DRG, NGF-IR, p38-IR, and BDNF-IR cells were increased for days 21. 
      In the SC, BDNF-IR, and p38-IR cells were increased from days 7 to 21. 
      CONCLUSION: In the DRG, NGF expression increased, mechanical thresholds were 
      reduced, and p38 and BDNF expression was increased in the NP group. p38 and BDNF 
      expression was increased in SC neurons during the same period. Inhibition of NGF 
      may be a potential treatment for neuropathic pain due to lumbar disc herniation. 
      LEVEL OF EVIDENCE: 5.
FAU - Seki, Shinobu
AU  - Seki S
AD  - Department of Orthopaedic Surgery, Fukushima Medical University School of 
      Medicine, Fukushima, Japan.
FAU - Sekiguchi, Miho
AU  - Sekiguchi M
FAU - Konno, Shin-Ichi
AU  - Konno SI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Spine (Phila Pa 1976)
JT  - Spine
JID - 7610646
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 107-73-3 (Phosphorylcholine)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Female
MH  - Ganglia, Spinal/*metabolism
MH  - Nerve Growth Factor/*metabolism
MH  - Neuralgia/*metabolism
MH  - Neurons/metabolism
MH  - Nucleus Pulposus/*metabolism
MH  - Pain Threshold/physiology
MH  - Phosphorylcholine
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2017/05/16 06:00
MHDA- 2019/06/20 06:00
CRDT- 2017/05/16 06:00
PHST- 2017/05/16 06:00 [pubmed]
PHST- 2019/06/20 06:00 [medline]
PHST- 2017/05/16 06:00 [entrez]
AID - 00007632-201801010-00004 [pii]
AID - 10.1097/BRS.0000000000002223 [doi]
PST - ppublish
SO  - Spine (Phila Pa 1976). 2018 Jan 1;43(1):E7-E15. doi: 
      10.1097/BRS.0000000000002223.