PMID- 28505182
OWN - NLM
STAT- MEDLINE
DCOM- 20170920
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 5
DP  - 2017
TI  - Using NextRAD sequencing to infer movement of herbivores among host plants.
PG  - e0177742
LID - 10.1371/journal.pone.0177742 [doi]
LID - e0177742
AB  - Herbivores often move among spatially interspersed host plants, tracking 
      high-quality resources through space and time. This dispersal is of particular 
      interest for vectors of plant pathogens. Existing molecular tools to track such 
      movement have yielded important insights, but often provide insufficient genetic 
      resolution to infer spread at finer spatiotemporal scales. Here, we explore the 
      use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer 
      movement of a highly-mobile winged insect, the potato psyllid (Bactericera 
      cockerelli), among host plants. The psyllid vectors the pathogen that causes 
      zebra chip disease in potato (Solanum tuberosum), but understanding and managing 
      the spread of this pathogen is limited by uncertainty about the insect's host 
      plant(s) outside of the growing season. We identified 1,978 polymorphic loci 
      among psyllids separated spatiotemporally on potato or in patches of bittersweet 
      nightshade (S. dulcumara), a weedy plant proposed to be the source of 
      potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic 
      similarity to insects on nightshade, consistent with regular movement between 
      these two host plants. However, a second subset of potato-collected psyllids was 
      genetically distinct from those collected on bittersweet nightshade; this 
      suggests that a currently unrecognized source, i.e., other nightshade patches or 
      a third host-plant species, could be contributing to psyllid populations in 
      potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine 
      scale in order to understand, predict, and manage disease spread. We demonstrate 
      that emerging sequencing technologies that detect genome-wide SNPs of a vector 
      can be used to infer such localized movement.
FAU - Fu, Zhen
AU  - Fu Z
AUID- ORCID: 0000-0002-8120-590X
AD  - Department of Entomology, Washington State University, Pullman, Washington, 
      United States of America.
FAU - Epstein, Brendan
AU  - Epstein B
AD  - School of Biological Sciences, Washington State University, Pullman, Washington, 
      United States of America.
FAU - Kelley, Joanna L
AU  - Kelley JL
AD  - School of Biological Sciences, Washington State University, Pullman, Washington, 
      United States of America.
FAU - Zheng, Qi
AU  - Zheng Q
AD  - Department of Bioinformatics and Biostatistics, University of Louisville, 
      Louisville, Kentucky, United States of America.
FAU - Bergland, Alan O
AU  - Bergland AO
AD  - Department of Biology, Stanford University, Stanford, California, United States 
      of America.
FAU - Castillo Carrillo, Carmen I
AU  - Castillo Carrillo CI
AD  - Department of Entomology, Washington State University, Pullman, Washington, 
      United States of America.
FAU - Jensen, Andrew S
AU  - Jensen AS
AD  - Northwest Potato Research Consortium, Lakeview, Oregon, United States of America.
FAU - Dahan, Jennifer
AU  - Dahan J
AD  - Department of Plant, Soil and Entomological Sciences, University of Idaho, 
      Moscow, Idaho, United States of America.
FAU - Karasev, Alexander V
AU  - Karasev AV
AD  - Department of Plant, Soil and Entomological Sciences, University of Idaho, 
      Moscow, Idaho, United States of America.
FAU - Snyder, William E
AU  - Snyder WE
AD  - Department of Entomology, Washington State University, Pullman, Washington, 
      United States of America.
LA  - eng
PT  - Journal Article
DEP - 20170515
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Computational Biology/methods
MH  - Hemiptera/classification/genetics
MH  - *Herbivory
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Host-Parasite Interactions
MH  - Host-Pathogen Interactions
MH  - Phylogeny
MH  - *Plant Diseases/microbiology/parasitology
MH  - *Plants
MH  - Solanum/parasitology
MH  - Solanum tuberosum/parasitology
PMC - PMC5432177
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/05/16 06:00
MHDA- 2017/09/21 06:00
PMCR- 2017/05/15
CRDT- 2017/05/16 06:00
PHST- 2016/11/20 00:00 [received]
PHST- 2017/05/02 00:00 [accepted]
PHST- 2017/05/16 06:00 [entrez]
PHST- 2017/05/16 06:00 [pubmed]
PHST- 2017/09/21 06:00 [medline]
PHST- 2017/05/15 00:00 [pmc-release]
AID - PONE-D-16-46089 [pii]
AID - 10.1371/journal.pone.0177742 [doi]
PST - epublish
SO  - PLoS One. 2017 May 15;12(5):e0177742. doi: 10.1371/journal.pone.0177742. 
      eCollection 2017.