PMID- 28505561
OWN - NLM
STAT- MEDLINE
DCOM- 20180312
LR  - 20180312
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 96
DP  - 2017 Oct 15
TI  - Class enzyme-based motors for "on the fly" enantiomer analysis of amino acids.
PG  - 275-280
LID - S0956-5663(17)30310-X [pii]
LID - 10.1016/j.bios.2017.04.051 [doi]
AB  - Here, two class-enzyme motors are properly designed allowing the rapid dispersion 
      of the class-enzyme D-amino acid oxidase (DAO) and L-amino acid oxidase (LAO) for 
      selective "on the fly" biodetection of D and L-amino acids (AAs), respectively. 
      The efficient movement together with the continuous release of fresh class-enzyme 
      leads to a greatly accelerated enzymatic reaction processes without the need of 
      external stirring or chemical and physical attachment of the enzyme. Ultra-fast 
      detection (<2min) and accurate quantifications of L-phenylalanine (L-Phe) in 
      plasma and whole-blood newborns samples diagnosed with Phenylketonuria and total 
      D-AAs in Vibrio cholera cultures are pioneer illustrated as relevant examples of 
      each enantiomer determination. These results opens clearly novel avenues in 
      biosensing for fast screening diagnostics, decentralized monitoring and design of 
      future points of care.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Garcia-Carmona, Laura
AU  - Garcia-Carmona L
AD  - Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, 
      Faculty of Biology, Environmental Sciences and Chemistry, University of Alcala, 
      28871 Alcala de Henares, Madrid, Spain.
FAU - Moreno-Guzman, Maria
AU  - Moreno-Guzman M
AD  - Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, 
      Faculty of Biology, Environmental Sciences and Chemistry, University of Alcala, 
      28871 Alcala de Henares, Madrid, Spain.
FAU - Gonzalez, Maria Cristina
AU  - Gonzalez MC
AD  - Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, 
      Faculty of Biology, Environmental Sciences and Chemistry, University of Alcala, 
      28871 Alcala de Henares, Madrid, Spain.
FAU - Escarpa, Alberto
AU  - Escarpa A
AD  - Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, 
      Faculty of Biology, Environmental Sciences and Chemistry, University of Alcala, 
      28871 Alcala de Henares, Madrid, Spain. Electronic address: 
      alberto.escarpa@uah.es.
LA  - eng
PT  - Journal Article
DEP - 20170506
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Amino Acids)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
RN  - EC 1.4.3.3 (D-Amino-Acid Oxidase)
SB  - IM
MH  - Amino Acids/*analysis/*blood
MH  - Biosensing Techniques/*methods
MH  - Cholera/microbiology
MH  - D-Amino-Acid Oxidase/chemistry
MH  - Humans
MH  - Infant, Newborn
MH  - L-Amino Acid Oxidase/chemistry
MH  - Phenylalanine/*analysis/*blood
MH  - Phenylketonurias/blood
MH  - Stereoisomerism
MH  - Vibrio cholerae/chemistry
OTO - NOTNLM
OT  - Class-enzyme motors
OT  - Enantiomers
OT  - Millimeter motors
OT  - Phenylketonuria
OT  - Vibrio Cholerae
EDAT- 2017/05/16 06:00
MHDA- 2018/03/13 06:00
CRDT- 2017/05/16 06:00
PHST- 2017/03/17 00:00 [received]
PHST- 2017/04/26 00:00 [revised]
PHST- 2017/04/29 00:00 [accepted]
PHST- 2017/05/16 06:00 [pubmed]
PHST- 2018/03/13 06:00 [medline]
PHST- 2017/05/16 06:00 [entrez]
AID - S0956-5663(17)30310-X [pii]
AID - 10.1016/j.bios.2017.04.051 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2017 Oct 15;96:275-280. doi: 10.1016/j.bios.2017.04.051. 
      Epub 2017 May 6.