PMID- 28506464
OWN - NLM
STAT- MEDLINE
DCOM- 20180322
LR  - 20181113
IS  - 1875-9777 (Electronic)
IS  - 1934-5909 (Print)
IS  - 1875-9777 (Linking)
VI  - 21
IP  - 1
DP  - 2017 Jul 6
TI  - Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following 
      Pneumonectomy.
PG  - 120-134.e7
LID - S1934-5909(17)30095-4 [pii]
LID - 10.1016/j.stem.2017.03.024 [doi]
AB  - To investigate the role of immune cells in lung regeneration, we used a 
      unilateral pneumonectomy model that promotes the formation of new alveoli in the 
      remaining lobes. Immunofluorescence and single-cell RNA sequencing found CD115+ 
      and CCR2+ monocytes and M2-like macrophages accumulating in the lung during the 
      peak of type 2 alveolar epithelial stem cell (AEC2) proliferation. Genetic loss 
      of function in mice and adoptive transfer studies revealed that bone 
      marrow-derived macrophages (BMDMs) traffic to the lung through a CCL2-CCR2 
      chemokine axis and are required for optimal lung regeneration, along with 
      Il4ra-expressing leukocytes. Our data suggest that these cells modulate AEC2 
      proliferation and differentiation. Finally, we provide evidence that group 2 
      innate lymphoid cells are a source of IL-13, which promotes lung regeneration. 
      Together, our data highlight the potential for immunomodulatory therapies to 
      stimulate alveologenesis in adults.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Lechner, Andrew J
AU  - Lechner AJ
AD  - Department of Anatomy, University of California, San Francisco, CA 94143, USA.
FAU - Driver, Ian H
AU  - Driver IH
AD  - Department of Anatomy, University of California, San Francisco, CA 94143, USA.
FAU - Lee, Jinwoo
AU  - Lee J
AD  - Department of Medicine and Howard Hughes Medical Institute, University of 
      California, San Francisco, CA 94143, USA.
FAU - Conroy, Carmen M
AU  - Conroy CM
AD  - Department of Anatomy, University of California, San Francisco, CA 94143, USA.
FAU - Nagle, Abigail
AU  - Nagle A
AD  - Department of Anatomy, University of California, San Francisco, CA 94143, USA.
FAU - Locksley, Richard M
AU  - Locksley RM
AD  - Department of Medicine and Howard Hughes Medical Institute, University of 
      California, San Francisco, CA 94143, USA.
FAU - Rock, Jason R
AU  - Rock JR
AD  - Department of Anatomy, University of California, San Francisco, CA 94143, USA. 
      Electronic address: rockjr@bu.edu.
LA  - eng
GR  - T32 GM007618/GM/NIGMS NIH HHS/United States
GR  - P01 HL107202/HL/NHLBI NIH HHS/United States
GR  - R01 HL127002/HL/NHLBI NIH HHS/United States
GR  - R01 HL128903/HL/NHLBI NIH HHS/United States
GR  - F30 HL131198/HL/NHLBI NIH HHS/United States
GR  - U01 HL134766/HL/NHLBI NIH HHS/United States
GR  - R37 AI026918/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170511
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Il4ra protein, mouse)
RN  - 0 (Interleukin-13)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
CIN - Stem Cell Investig. 2018 Mar 31;5:8. PMID: 29682515
MH  - Animals
MH  - Interleukin-13/genetics/immunology
MH  - Lung/*physiology
MH  - Macrophages, Alveolar/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Monocytes/*immunology
MH  - *Pneumonectomy
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/immunology
MH  - Receptors, CCR2/genetics/immunology
MH  - Receptors, Cell Surface/genetics/immunology
MH  - Regeneration/genetics/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC5501755
MID - NIHMS864448
OTO - NOTNLM
OT  - ILC2
OT  - lung regeneration
OT  - macrophage
OT  - monocyte
OT  - pneumonectomy
OT  - type 2 alveolar pneumocyte
EDAT- 2017/05/17 06:00
MHDA- 2018/03/23 06:00
PMCR- 2018/07/06
CRDT- 2017/05/17 06:00
PHST- 2016/08/19 00:00 [received]
PHST- 2017/02/09 00:00 [revised]
PHST- 2017/03/27 00:00 [accepted]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2018/03/23 06:00 [medline]
PHST- 2017/05/17 06:00 [entrez]
PHST- 2018/07/06 00:00 [pmc-release]
AID - S1934-5909(17)30095-4 [pii]
AID - 10.1016/j.stem.2017.03.024 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2017 Jul 6;21(1):120-134.e7. doi: 10.1016/j.stem.2017.03.024. 
      Epub 2017 May 11.