PMID- 285073
OWN - NLM
STAT- MEDLINE
DCOM- 19790611
LR  - 20151119
IS  - 0301-3952 (Print)
IS  - 0301-3952 (Linking)
VI  - 7
IP  - 1
DP  - 1979 Mar
TI  - Distribution patterns of DNA template activity in the embryonic tooth organ: an 
      acridine orange ultracytochemical study.
PG  - 37-48
AB  - The ultracytochemical acridine orange (AO) method has been employed to 
      demonstrate DNA template activity within embryonic incisor tooth organs excised 
      from New Zealand White rabbits during the 25th day of gestation. Survey 
      ultrastructural examination revealed characteristic distribution patterns of AO 
      positive cell populations in the inner and outer enamel epithelium and the 
      adjacent ectomesenchyme of the cervical loop region and in populations of 
      fibroblast-like cells connecting as a band the outer AO positive cell groups. 
      With increasing differentiation of ameloblasts and odontoblasts the number of AO 
      positive cells as well as the number of AO chromatin interaction products per 
      single cell nucleus decreased. Treatment with RNase prior to incubation with AO 
      did not result in a noticeable loss of AO chromatin interaction products 
      suggesting that RNA is negligible as a target for AO. The present results and 
      comparison with other biological system indicated that interaction of AO with DNA 
      sites coding for RNA appears to be restricted to specific templates in particular 
      physiological conditions, e.g., stimulation of cells prior to differentiation by 
      extracellular factors.
FAU - Lehmann, R
AU  - Lehmann R
LA  - eng
PT  - Journal Article
PL  - France
TA  - J Biol Buccale
JT  - Journal de biologie buccale
JID - 0400336
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Nucleus/metabolism
MH  - Chromatin/metabolism
MH  - DNA/*metabolism
MH  - Ectoderm/ultrastructure
MH  - Epithelium/ultrastructure
MH  - Incisor/embryology/metabolism/ultrastructure
MH  - Mesoderm/ultrastructure
MH  - *Odontogenesis
MH  - Rabbits
MH  - Templates, Genetic
MH  - Tooth Germ/metabolism/*ultrastructure
MH  - Transcription, Genetic
EDAT- 1979/03/01 00:00
MHDA- 1979/03/01 00:01
CRDT- 1979/03/01 00:00
PHST- 1979/03/01 00:00 [pubmed]
PHST- 1979/03/01 00:01 [medline]
PHST- 1979/03/01 00:00 [entrez]
PST - ppublish
SO  - J Biol Buccale. 1979 Mar;7(1):37-48.