PMID- 28507614
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1857-9655 (Print)
IS  - 1857-9655 (Electronic)
IS  - 1857-9655 (Linking)
VI  - 5
IP  - 2
DP  - 2017 Apr 15
TI  - Functional Pattern of Increasing Concentrations of Brain-Derived Neurotrophic 
      Factor in Spiral Ganglion: Implications for Research on Cochlear Implants.
PG  - 121-125
LID - 10.3889/oamjms.2017.017 [doi]
AB  - BACKGROUND: As previously various studies have suggested application of 
      brain-derived neurotrophic factor (BDNF) may be considered as a promising future 
      therapy for hearing deficits, in particular for the improvement of cochlear 
      neurone loss during cochlear implantation. AIM: The present study's aim was to 
      establish the upper threshold of the concentration of BDNF in Naval Medical 
      Research Institute (NMRI) mice spiral ganglion outgrowth. METHODS: Spiral 
      ganglion explants were prepared from post-natal day 4 (p4) (NMRI) mice of both 
      sexes under the approval and guidelines of the regional council of Hearing 
      Research Institute Tubingen. RESULTS: Spiral ganglion explants were cultured at 
      postnatal days 4 in the presence of different concentrations of BDNF as described 
      under methods. We chose an age of postnatal day (P4) and concentrations of BDNF 
      0; 6; 12.5; 25 and 50 ng/ml. Averaged neurite outgrowth is measured in 4 
      different cultures that were treated with different concentrations. Results show 
      that with increasing concentrations of BDNF, the neurite density increases. 
      CONCLUSION: The present finding show evidence that BDNF has a clear incremental 
      effect on the number of neurites of spiral ganglia in the prehearing organ, but 
      less on the neurite length. The upper threshold of exogenous BNDF concentration 
      on spiral ganglion explant is 25 ng/ml. This means that concentration beyond this 
      level has no further incremental impact. Therefore our suggestion for hydrogel 
      concentration in NMRA mice in future research should be 25 ng/ml.
FAU - Ramku, Emina
AU  - Ramku E
AD  - University Clinical Center, Prishtina, Kosovo.
FAU - Ramku, Refik
AU  - Ramku R
AD  - Private Polyclinic OTOKIRURGJIA, Prishtina, Kosovo.
FAU - Spanca, Dugagjin
AU  - Spanca D
AD  - University Clinical Center - Audiology, Prishtina, Kosovo.
FAU - Zhjeqi, Valbona
AU  - Zhjeqi V
AD  - University Clinical Center, Prishtina, Kosovo.
LA  - eng
PT  - Journal Article
DEP - 20170227
PL  - North Macedonia
TA  - Open Access Maced J Med Sci
JT  - Open access Macedonian journal of medical sciences
JID - 101662294
PMC - PMC5420760
OTO - NOTNLM
OT  - Animal experiments
OT  - BDNF
OT  - in vitro techniques
OT  - outgrowth
OT  - spiral ganglion
EDAT- 2017/05/17 06:00
MHDA- 2017/05/17 06:01
PMCR- 2017/02/27
CRDT- 2017/05/17 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/02/22 00:00 [revised]
PHST- 2017/02/25 00:00 [accepted]
PHST- 2017/05/17 06:00 [entrez]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2017/05/17 06:01 [medline]
PHST- 2017/02/27 00:00 [pmc-release]
AID - OAMJMS-5-121 [pii]
AID - 10.3889/oamjms.2017.017 [doi]
PST - epublish
SO  - Open Access Maced J Med Sci. 2017 Feb 27;5(2):121-125. doi: 
      10.3889/oamjms.2017.017. eCollection 2017 Apr 15.