PMID- 28507940
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2228-5881 (Print)
IS  - 2251-7308 (Electronic)
IS  - 2228-5881 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Apr
TI  - Cord Blood Cells Responses to IL2, IL7 and IL15 Cytokines for mTOR Expression.
PG  - 81-85
LID - 10.15171/apb.2017.010 [doi]
AB  - Purpose: Mammalian target of rapamycin (mTOR)is important in hematopoiesis and 
      affect cell growth,differentiation and survival. Although previous studies were 
      identified the effect of cytokines on the mononuclear cells development however 
      the cytokines effect on mTOR in cord blood mononuclear cells was unclear. The aim 
      of this study was to evaluate mTOR expression in cord blood mononuclear and cord 
      blood stem cells (CD34(+) cells) in culture conditions for lymphoid cell 
      development. Methods: Isolation of The mononuclear cells (MNCs) from umbilical 
      cord blood were done with use of Ficollpaque density gradient. We evaluated 
      cultured cord blood mononuclear and CD34(+) cells in presece of IL2, IL7 and IL15 
      at distinct time points during 21 days by using flow cytometry. In this study, we 
      presented the role of IL2, IL7 and IL15 on the expression of mTOR in cord blood 
      cells. Results: mTOR expression were increased in peresence of IL2, IL7 and IL15 
      in day 14 and afterword reduced. However in persence of IL2 and IL15 expression 
      of mTOR significantly reduced. mTOR expression in CD34(+) cells decreased 
      significantly from day7 to day 21 in culture. Conclusion: cytokines play 
      important role in mTOR expression during hematopoiesis and development of cord 
      blood mononuclear cells.
FAU - Mohammadian, Anahita
AU  - Mohammadian A
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Naderali, Elahe
AU  - Naderali E
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Mohammadi, Seyedeh Momeneh
AU  - Mohammadi SM
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Movasaghpour, Aliakbar
AU  - Movasaghpour A
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Valipour, Behnaz
AU  - Valipour B
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Nouri, Mohammad
AU  - Nouri M
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
FAU - Nozad Charoudeh, Hojjatollah
AU  - Nozad Charoudeh H
AD  - Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - Iran
TA  - Adv Pharm Bull
JT  - Advanced pharmaceutical bulletin
JID - 101578021
PMC - PMC5426737
OTO - NOTNLM
OT  - Cord blood
OT  - Cytokines
OT  - mTOR
EDAT- 2017/05/17 06:00
MHDA- 2017/05/17 06:01
PMCR- 2017/04/01
CRDT- 2017/05/17 06:00
PHST- 2016/08/15 00:00 [received]
PHST- 2017/01/22 00:00 [revised]
PHST- 2017/01/24 00:00 [accepted]
PHST- 2017/05/17 06:00 [entrez]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2017/05/17 06:01 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 10.15171/apb.2017.010 [doi]
PST - ppublish
SO  - Adv Pharm Bull. 2017 Apr;7(1):81-85. doi: 10.15171/apb.2017.010. Epub 2017 Apr 
      13.