PMID- 28507952 OWN - NLM STAT- MEDLINE DCOM- 20171227 LR - 20201209 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 7 DP - 2017 TI - Comparison of Growth and the Cytokines Induced by Pathogenic Yersinia enterocolitica Bio-Serotypes 3/O: 3 and 2/O: 9. PG - 158 LID - 10.3389/fcimb.2017.00158 [doi] LID - 158 AB - Pathogenic Yersinia enterocolitica is widely distributed in China where the primary bio-serotypes are 3/O: 3 and 2/O: 9. Recently, the distribution of 2/O: 9 strains are being gradually replaced by 3/O: 3 strains where presently 3/O: 3 strains are the major pathogenic Y. enterocolitica in China. To identify the growth conditions and cytokines induced by Y. enterocolitica and providing some clues for this shift, we performed competitive growth in vitro and in vivo for these two bio-serotype strains; and we also compared the cytokines induced by them in infected BALB/C mice. We found 2/O: 9 strains grew more in vitro, while 3/O: 3 strains grew more in vivo regardless of using single cultures or mixed cultures. The cytokines induced by the two strains were similar: interleukin-6 (IL-6), IL-9, IL-13, granulocyte colony-stimulating factor (G-CSF), chemokines (KC), monocyte chemotactic protein 1 (MCP-1), macrophage inflammation protein-1alpha (MIP-1alpha), tumor necrosis factor-alpha (TNF-alpha), and RANTES were statistically up-regulated upon activation of normal T cells compared to the control. The cytokine values were higher in mixed infections than in single infections except for IL-6, G-CSF, and KC. The data illustrated the different growth of pathogenic Y. enterocolitica bio-serotype 3/O: 3 and 2/O: 9 in vitro and in vivo, and the cytokine changes induced by the two strains in infected BALB/C mice. The growth comparisons of two strains maybe reflect the higher pathogenic ability or resistance to host immune response for Y. enterocolitica bio-serotype 3/O: 3 and maybe it as one of the reason for bacteria shift. FAU - Yang, Haoshu AU - Yang H AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. AD - Lianyungang Center of Disease Control and PreventionLianyungang, China. FAU - Gu, Wenpeng AU - Gu W AD - Yunnan Provincial Centres for Disease Control and PreventionKunming, China. FAU - Qiu, Haiyan AU - Qiu H AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Sun, Guixiang AU - Sun G AD - Department of Public Health, Xuzhou Medical CollegeXuzhou, China. FAU - Liang, Junrong AU - Liang J AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Li, Kewei AU - Li K AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Xiao, Yuchun AU - Xiao Y AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Duan, Ran AU - Duan R AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Jing, Huaiqi AU - Jing H AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. FAU - Wang, Xin AU - Wang X AD - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China. LA - eng PT - Journal Article DEP - 20170501 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 RN - 0 (Chemokine CCL2) RN - 0 (Chemokine CCL5) RN - 0 (Chemokines) RN - 0 (Cytokines) RN - 0 (Interleukin-13) RN - 0 (Interleukin-6) RN - 0 (Interleukin-9) RN - 143011-72-7 (Granulocyte Colony-Stimulating Factor) SB - IM MH - Animals MH - Chemokine CCL2/metabolism MH - Chemokine CCL5/metabolism MH - Chemokines/metabolism MH - China MH - Cytokines/blood/*metabolism MH - Disease Models, Animal MH - Female MH - Granulocyte Colony-Stimulating Factor/metabolism MH - Interleukin-13/metabolism MH - Interleukin-6/metabolism MH - Interleukin-9/metabolism MH - Mice MH - Mice, Inbred BALB C MH - *Serogroup MH - T-Lymphocytes MH - Virulence/genetics MH - Yersinia Infections/*immunology/*microbiology MH - Yersinia enterocolitica/classification/*growth & development/*pathogenicity PMC - PMC5410558 OTO - NOTNLM OT - Yersinia enterocolitica OT - bio-serotypes 2/O: 9 OT - bio-serotypes 3/O: 3 OT - competitive inhibition OT - cytokines EDAT- 2017/05/17 06:00 MHDA- 2017/12/28 06:00 PMCR- 2017/01/01 CRDT- 2017/05/17 06:00 PHST- 2017/02/20 00:00 [received] PHST- 2017/04/12 00:00 [accepted] PHST- 2017/05/17 06:00 [entrez] PHST- 2017/05/17 06:00 [pubmed] PHST- 2017/12/28 06:00 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - 10.3389/fcimb.2017.00158 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2017 May 1;7:158. doi: 10.3389/fcimb.2017.00158. eCollection 2017.