PMID- 28508449
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20181202
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 47
IP  - 7
DP  - 2017 Jul
TI  - Satellite glial cells in human trigeminal ganglia have a broad expression of 
      functional Toll-like receptors.
PG  - 1181-1187
LID - 10.1002/eji.201746989 [doi]
AB  - Toll-like receptors (TLRs) orchestrate immune responses to a wide variety of 
      danger- and pathogen-associated molecular patterns. Compared to the central 
      nervous system (CNS), expression profile and function of TLRs in the human 
      peripheral nervous system (PNS) are ill-defined. We analyzed TLR expression of 
      satellite glial cells (SGCs) and microglia, glial cells predominantly involved in 
      local immune responses in ganglia of the human PNS and normal-appearing white 
      matter (NAWM) of the CNS, respectively. Ex vivo flow cytometry analysis of cell 
      suspensions obtained from human cadaveric trigeminal ganglia (TG) and NAWM showed 
      that both SGCs and microglia expressed TLR1-5, TLR7, and TLR9, although 
      expression levels varied between these cell types. Immunohistochemistry confirmed 
      expression of TLR1-TLR4 and TLR9 by SGCs in situ. Stimulation of TG- and 
      NAWM-derived cell suspensions with ligands of TLR1-TLR6, but not TLR7 and TLR9, 
      induced interleukin 6 (IL-6) secretion. We identified CD45(LOW) CD14(POS) SGCs 
      and microglia, but not CD45(HIGH) leukocytes and CD45(NEG) cells as the main 
      source of IL-6 and TNF-alpha upon stimulation with TLR3 and TLR5 ligands. In 
      conclusion, human TG-resident SGCs express a broad panel of functional TLRs, 
      suggesting their role in initiating and orchestrating inflammation to pathogens 
      in human sensory ganglia.
CI  - (c) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Mitterreiter, Johanna G
AU  - Mitterreiter JG
AD  - Research Center for Emerging Infections and Zoonoses, University of Veterinary 
      Medicine Hannover, Germany.
AD  - Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - Ouwendijk, Werner J D
AU  - Ouwendijk WJD
AD  - Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - van Velzen, Monique
AU  - van Velzen M
AD  - Department of Anesthesiology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - van Nierop, Gijsbert P
AU  - van Nierop GP
AD  - Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
AD  - Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - Osterhaus, Albert D M E
AU  - Osterhaus ADME
AD  - Research Center for Emerging Infections and Zoonoses, University of Veterinary 
      Medicine Hannover, Germany.
FAU - Verjans, Georges M G M
AU  - Verjans GMGM
AUID- ORCID: 0000-0002-2465-2674
AD  - Research Center for Emerging Infections and Zoonoses, University of Veterinary 
      Medicine Hannover, Germany.
AD  - Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20170601
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Cytokines)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 7)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Humans
MH  - Interleukin-6/immunology/metabolism
MH  - Microglia/*immunology/metabolism
MH  - Neuroglia/*immunology/metabolism
MH  - Signal Transduction
MH  - Toll-Like Receptor 3/genetics/immunology/*metabolism
MH  - Toll-Like Receptor 4/genetics/immunology/*metabolism
MH  - Toll-Like Receptor 7/genetics/immunology/*metabolism
MH  - Toll-Like Receptors/genetics/immunology/*metabolism
MH  - Trigeminal Ganglion/cytology/immunology
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
MH  - White Matter/cytology/immunology
OTO - NOTNLM
OT  - Cytokines
OT  - Human trigeminal ganglion
OT  - Microglia
OT  - Satellite glial cell
OT  - Toll-like receptor
EDAT- 2017/05/17 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/05/17 06:00
PHST- 2017/02/09 00:00 [received]
PHST- 2017/04/04 00:00 [revised]
PHST- 2017/05/10 00:00 [accepted]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/05/17 06:00 [entrez]
AID - 10.1002/eji.201746989 [doi]
PST - ppublish
SO  - Eur J Immunol. 2017 Jul;47(7):1181-1187. doi: 10.1002/eji.201746989. Epub 2017 
      Jun 1.