PMID- 28509878
OWN - NLM
STAT- MEDLINE
DCOM- 20180123
LR  - 20181113
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 9
IP  - 5
DP  - 2017 May 16
TI  - Antigenic and Biological Characterization of ORF2-6 Variants at Early Times 
      Following PRRSV Infection.
LID - 10.3390/v9050113 [doi]
LID - 113
AB  - Genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) 
      challenges efforts to develop effective and broadly acting vaccines. Although 
      genetic variation in PRRSV has been extensively documented, the effects of this 
      variation on virus phenotype are less well understood. In the present study, 
      PRRSV open reading frame (ORF)2-6 variants predominant during the first six weeks 
      following experimental infection were characterized for antigenic and replication 
      phenotype. There was limited genetic variation during these early times after 
      infection; however, distinct ORF2-6 haplotypes that differed from the NVSL97-7895 
      inoculum were identified in each of the five pigs examined. Chimeric viruses 
      containing all or part of predominant ORF2-6 haplotypes were constructed and 
      tested in virus neutralization and in vitro replication assays. In two pigs, 
      genetic variation in ORF2-6 resulted in increased resistance to neutralization by 
      autologous sera. Mapping studies indicated that variation in either ORF2-4 or 
      ORF5-6 could confer increased neutralization resistance, but there was no single 
      amino acid substitution that was predictive of neutralization phenotype. Detailed 
      analyses of the early steps in PRRSV replication in the presence and absence of 
      neutralizing antibody revealed both significant inhibition of virion attachment 
      and, independently, a significant delay in the appearance of newly synthesized 
      viral RNA. In all pigs, genetic variation in ORF2-6 also resulted in significant 
      reduction in infectivity on MARC-145 cells, suggesting variation in ORF2-6 may 
      also be important for virus replication in vivo. Together, these data reveal that 
      variation appearing early after infection, though limited, alters important virus 
      phenotypes and contributes to antigenic and biologic diversity of PRRSV.
FAU - Evans, Alyssa B
AU  - Evans AB
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      alyssa.ben.evans@gmail.com.
FAU - Loyd, Hyelee
AU  - Loyd H
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      heri1008@iastate.edu.
FAU - Dunkelberger, Jenelle R
AU  - Dunkelberger JR
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      jenelled@iastate.edu.
FAU - van Tol, Sarah
AU  - van Tol S
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      savantol@utmb.edu.
FAU - Bolton, Marcus J
AU  - Bolton MJ
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      mjohnbolton@gmail.com.
FAU - Dorman, Karin S
AU  - Dorman KS
AD  - Departments of Statistics and Genetics, Development and Cell Biology, Iowa State 
      University, Ames, IA 50011, USA. kdorman@iastate.edu.
FAU - Dekkers, Jack C M
AU  - Dekkers JCM
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      jdekkers@iastate.edu.
FAU - Carpenter, Susan
AU  - Carpenter S
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011, USA. 
      scarp@iastate.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170516
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antigenic Variation/*genetics/*immunology
MH  - Antigens, Viral/genetics/immunology
MH  - Base Sequence
MH  - Cell Line
MH  - Disease Models, Animal
MH  - *Genetic Variation
MH  - Open Reading Frames/*genetics/*immunology
MH  - Phenotype
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - RNA, Viral/genetics
MH  - Sus scrofa
MH  - Swine
MH  - Virion
MH  - Virus Attachment
MH  - Virus Replication
PMC - PMC5454425
OTO - NOTNLM
OT  - PRRSV
OT  - antigenic variation
OT  - genetic diversity
OT  - neutralization
OT  - replication phenotype
COIS- The authors declare no conflict of interest.
EDAT- 2017/05/17 06:00
MHDA- 2018/01/24 06:00
PMCR- 2017/05/01
CRDT- 2017/05/17 06:00
PHST- 2017/03/08 00:00 [received]
PHST- 2017/04/18 00:00 [revised]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/05/17 06:00 [entrez]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2018/01/24 06:00 [medline]
PHST- 2017/05/01 00:00 [pmc-release]
AID - v9050113 [pii]
AID - viruses-09-00113 [pii]
AID - 10.3390/v9050113 [doi]
PST - epublish
SO  - Viruses. 2017 May 16;9(5):113. doi: 10.3390/v9050113.