PMID- 28511131
OWN - NLM
STAT- MEDLINE
DCOM- 20180518
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 115
DP  - 2017 Dec
TI  - Genetic diversity and phylogenetic analysis of the ORF5 gene of PRRSV from 
      central China.
PG  - 226-234
LID - S0034-5288(16)30322-8 [pii]
LID - 10.1016/j.rvsc.2017.05.013 [doi]
AB  - To more fully understand the genetic diversity and molecular epidemiology of 
      prevailing porcine reproductive and respiratory syndrome virus (PRRSV) in Henan 
      province of China, 112 full-length ORF5 gene sequences, originating from Henan 
      province between 2006 and 2015, were subjected to sequence variation and 
      phylogenetic analysis. Phylogenetic analysis revealed that all Henan isolates 
      belonged to the Type 2 genotype and could be further divided into three 
      subgroups. Subgroup 1 and 2 viruses predominated in Henan and subgroup 2 overtook 
      subgroup 1 as the most prevalent PRRSV between 2006 and 2015. Highly pathogenic 
      PRRSV (HP-PRRSV) isolates predominated in Henan and eight RespPRRSV MLV 
      vaccine-like isolates were observed in subgroup 3. Sequence variation analysis 
      revealed that the ORF5 genes of all Henan isolates shared >83.3% nucleotide and 
      >80.1% amino acid sequence identity with each other. Primary neutralizing epitope 
      (PNE) analysis revealed that, relative to the attenuated RespPRRSV MLV vaccine 
      isolate, all but one of the subgroup 1 Henan isolates had mutations at amino acid 
      39 within the key PNE of GP5. Analysis of the immunoreceptor tyrosine-based 
      inhibitory motif (ITIM) in GP5 revealed that all but two of the Henan isolates 
      had a highly conserved sequence between amino acids 77 and 82 positions of GP5. 
      N-linked glycosylation site (NGS) analysis revealed a novel potential NGS at GP5 
      amino acid position 59 in two of the subgroup 2 Henan isolates. Another novel GP5 
      amino acid mutation ((44)N-->(44)D) was found in a single subgroup 1 Henan isolate 
      (HeNan-A9) in a glycosylation site that is known to be crucial for PRRSV 
      infectivity.
CI  - Copyright (c) 2017. Published by Elsevier Ltd.
FAU - Zhang, Liujun
AU  - Zhang L
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China.
FAU - Feng, Yan
AU  - Feng Y
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China.
FAU - Martin, Darren P
AU  - Martin DP
AD  - Institute of Infectious Diseases and Molecular Medicine, Computational Biology 
      Group, University of Cape Town, Cape Town 7549, South Africa.
FAU - Chen, Jing
AU  - Chen J
AD  - College of Animal Science and Veterinary Medicine, Jilin University, Changchun 
      130062, China.
FAU - Ma, Sixu
AU  - Ma S
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China.
FAU - Xia, Pingan
AU  - Xia P
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China. Electronic address: xpa88@163.com.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou 450002, China. Electronic address: zhanggaiping2003@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China/epidemiology
MH  - *Genetic Variation
MH  - Genotype
MH  - Molecular Epidemiology
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Sequence Alignment
MH  - Swine
MH  - Viral Envelope Proteins/chemistry/*genetics/metabolism
OTO - NOTNLM
OT  - Genetic diversity
OT  - Henan province
OT  - ORF5
OT  - PRRSV
OT  - Phylogenetic analysis
EDAT- 2017/05/17 06:00
MHDA- 2018/05/19 06:00
CRDT- 2017/05/17 06:00
PHST- 2016/09/13 00:00 [received]
PHST- 2017/05/09 00:00 [revised]
PHST- 2017/05/10 00:00 [accepted]
PHST- 2017/05/17 06:00 [pubmed]
PHST- 2018/05/19 06:00 [medline]
PHST- 2017/05/17 06:00 [entrez]
AID - S0034-5288(16)30322-8 [pii]
AID - 10.1016/j.rvsc.2017.05.013 [doi]
PST - ppublish
SO  - Res Vet Sci. 2017 Dec;115:226-234. doi: 10.1016/j.rvsc.2017.05.013. Epub 2017 May 
      11.