PMID- 28511862
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20180319
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 182
DP  - 2017 Jul
TI  - Effect of different concentrations of egg yolk and virgin coconut oil in 
      Tris-based extenders on chilled and frozen-thawed bull semen.
PG  - 21-27
LID - S0378-4320(16)30613-3 [pii]
LID - 10.1016/j.anireprosci.2017.03.024 [doi]
AB  - The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) 
      combined with different percentages of egg yolk in Tris extender on the quality 
      of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls 
      were collected using an electroejaculator. Semen samples were diluted with 8% VCO 
      in Tris extender which contained different concentrations 0% (control), 4%, 8%, 
      12%, 16% and 20% egg yolk. The diluted semen samples were divided into two 
      fractions: one was chilled and stored at 4 degrees C until evaluation after 24, 72, and 
      144h; the second fraction was processed by chilling for 3h at 4 degrees C to equilibrate, 
      then packaged in 0.25ml straws and frozen and stored in liquid nitrogen at -196 degrees C 
      until evaluation after 7 and 14 days. Both chilled and frozen semen samples were 
      then thawed at 37 degrees C and assessed for general motility using computer-assisted 
      semen analysis (CASA), viability, acrosome integrity, and morphology 
      (eosin-nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid 
      peroxidation (thiobarbituric acid-reactive substances (TBARS)). The results 
      indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater 
      sperm quality (P<0.05) as compared with the control. The treatment with 20% egg 
      yolk had the greatest sperm quality (P<0.05) among the treated groups for both 
      chilled and frozen-thawed semen. In conclusion, the use of 8% VCO combined with 
      20% egg yolk in a Tris-based extender enhanced the values for chilled and 
      frozen-thawed quality variables of bull sperm.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Tarig, A A
AU  - Tarig AA
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia; 
      Department of Dairy Production, Faculty of Animal Production, University of 
      Khartoum, Sudan.
FAU - Wahid, H
AU  - Wahid H
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia. 
      Electronic address: wahidh@upm.edu.my.
FAU - Rosnina, Y
AU  - Rosnina Y
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Yimer, N
AU  - Yimer N
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Goh, Y M
AU  - Goh YM
AD  - Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Baiee, F H
AU  - Baiee FH
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Khumran, A M
AU  - Khumran AM
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Salman, H
AU  - Salman H
AD  - Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
FAU - Ebrahimi, M
AU  - Ebrahimi M
AD  - Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20170410
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Polyenes)
RN  - 0 (dimethyl all-(E)-octacosa-10,12,14,16,18-pentaenedioate)
RN  - Q9L0O73W7L (Coconut Oil)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Membrane
MH  - Coconut Oil/*chemistry
MH  - Cryopreservation/veterinary
MH  - Cryoprotective Agents/chemistry/*pharmacology
MH  - Egg Yolk/*chemistry
MH  - Male
MH  - Polyenes
MH  - Semen/*physiology
MH  - Semen Preservation/methods/*veterinary
MH  - Sperm Motility/drug effects
MH  - Spermatozoa/drug effects
OTO - NOTNLM
OT  - Bull semen
OT  - Chilling
OT  - Cryopreservation
OT  - Egg yolk
OT  - Extender
OT  - Virgin coconut oil
EDAT- 2017/05/18 06:00
MHDA- 2018/03/20 06:00
CRDT- 2017/05/18 06:00
PHST- 2016/11/04 00:00 [received]
PHST- 2017/03/31 00:00 [accepted]
PHST- 2017/05/18 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2017/05/18 06:00 [entrez]
AID - S0378-4320(16)30613-3 [pii]
AID - 10.1016/j.anireprosci.2017.03.024 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2017 Jul;182:21-27. doi: 10.1016/j.anireprosci.2017.03.024. Epub 
      2017 Apr 10.