PMID- 28512471
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220330
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2017
DP  - 2017
TI  - Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem 
      Cells into Neuron-Like Cells.
PG  - 1651325
LID - 10.1155/2017/1651325 [doi]
LID - 1651325
AB  - Objective. Human umbilical cord mesenchymal stem cells (hUC-MSCs) potentially 
      differentiate to various types of cells including neuron-like cells. The natural 
      polyphenol resveratrol benefits patients with many diseases including ischemic 
      brain injury. We hypothesize that resveratrol induces differentiation of hUC-MSCs 
      into neuron-like cells. Methods. Flow cytometry was used to determine the surface 
      antigens in different stage of hUC-MSCs (P2, P5, and P10). Nestin, 
      neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP) were 
      detected by immunocytochemistry, Western blotting, and real time RT-PCT. The 
      cultured hUC-MSCs were treated with resveratrol at different concentrations (0, 
      7.5, 15.0, and 30.0 mg/L). Nestin, GFAP, and NSE protein and mRNA were measured 
      at posttreatment time points of 2 h, 4 h, 6 h, 12 h, and 24 h. Results. 
      Neuron-like cells were found in hUC-MSCs treated by resveratrol at concentrations 
      of 15.0 and 30.0 mg/L, but not in hUC-MSCs treated with vehicle and 7.5 mg/L 
      resveratrol. Furthermore, immunocytochemical staining revealed that nestin and 
      NSE immunoreactivities were positive in resveratrol-treated hUC-MSCs at 
      concentrations of 15.0 and 30.0 mg/L. Resveratrol treatment significantly 
      increased nestin and NSE protein and mRNA levels 4 h after the treatment. 
      However, resveratrol treatment did not change GFAP immunoreactivities and protein 
      and mRNA expression levels in cultured hUC-MSCs. Conclusions. Taken together, 
      resveratrol treatment induces a differentiation of hUC-MSCs into neuron-like 
      cells at relatively high concentrations.
FAU - Guo, Li
AU  - Guo L
AD  - Department of Neurosurgery, The Second Hospital of Hebei Medical University, 
      Shijiazhuang, Hebei 050000, China.
AD  - Department of Pharmacy, The Fourth Hospital of Hebei Medical University, 
      Shijiazhuang, Hebei 050011, China.
FAU - Wang, Liang
AU  - Wang L
AD  - Department of Neurosurgery, The Second Hospital of Hebei Medical University, 
      Shijiazhuang, Hebei 050000, China.
AD  - North China University of Science and Technology Affiliated Hospital, Tangshan, 
      Hebei 63000, China.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Anesthesiology and Critical Care, The University of Texas MD 
      Anderson Cancer Center, Houston, TX 77030, USA.
FAU - Yun-Peng, Shi
AU  - Yun-Peng S
AD  - Department of Neurosurgery, The Second Hospital of Hebei Medical University, 
      Shijiazhuang, Hebei 050000, China.
FAU - Zhou, Jing-Jing
AU  - Zhou JJ
AD  - Department of Anesthesiology and Critical Care, The University of Texas MD 
      Anderson Cancer Center, Houston, TX 77030, USA.
FAU - Zhao, Zongmao
AU  - Zhao Z
AUID- ORCID: 0000-0002-2503-8684
AD  - Department of Neurosurgery, The Second Hospital of Hebei Medical University, 
      Shijiazhuang, Hebei 050000, China.
FAU - Li, De-Pei
AU  - Li DP
AD  - Department of Anesthesiology and Critical Care, The University of Texas MD 
      Anderson Cancer Center, Houston, TX 77030, USA.
LA  - eng
PT  - Journal Article
DEP - 20170420
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5415670
EDAT- 2017/05/18 06:00
MHDA- 2017/05/18 06:01
PMCR- 2017/04/20
CRDT- 2017/05/18 06:00
PHST- 2016/11/04 00:00 [received]
PHST- 2017/01/29 00:00 [revised]
PHST- 2017/02/15 00:00 [accepted]
PHST- 2017/05/18 06:00 [entrez]
PHST- 2017/05/18 06:00 [pubmed]
PHST- 2017/05/18 06:01 [medline]
PHST- 2017/04/20 00:00 [pmc-release]
AID - 10.1155/2017/1651325 [doi]
PST - ppublish
SO  - Stem Cells Int. 2017;2017:1651325. doi: 10.1155/2017/1651325. Epub 2017 Apr 20.