PMID- 2851256
OWN - NLM
STAT- MEDLINE
DCOM- 19890301
LR  - 20190903
IS  - 0001-6632 (Print)
IS  - 0001-6632 (Linking)
VI  - 38
IP  - 6
DP  - 1988 Jun
TI  - Electron microscopic localization of acridine orange chromatin interaction 
      products in cells transformed by herpes simplex virus type 2.
PG  - 693-704
AB  - The purpose of this study was to examine the distribution pattern of acridine 
      orange (AO) binding to DNA in transformed cells and derived tumor cells. Two cell 
      clones from hamster embryonic fibroblasts (HEF) transformed by herpes simplex 
      virus type 2, tumor cells derived from the transformed cell clones, and normal 
      HEF in culture were processed for ultracytochemistry. Uptake studies and 
      autoradiography using [3H] thymidine and [3H]-uridine were also performed. 
      Ultrastructural examination revealed that AO became bound to DNA exclusively 
      within the euchromatin portion of the cell nucleus. The percentages of 
      AO-positive cell nuclei were 18.0% and 15.5% in transformed cell clones and 21.3% 
      and 18.3% in the derived tumor cells, but only 2. 7% in the HEF. The average 
      amounts of AO-chromatin interaction products increased in the transformed and 
      tumor cells. Labeling indices of [3H] thymidine were 28.9% and 33.2% in both the 
      transformed cell clones, 32.4% and 36.9% in their tumor cells, and 3.7% in the 
      HEF. Euchromatin/heterochromatin (EU/HEF) ratios were found to be higher in 
      AO-positive nuclei than in AO-negative nuclei in each cell line. The increase in 
      the number of AO-positive nuclei and in the EU/HET ratios may represent a basic 
      reaction of cell nuclei in transformation and tumorigenesis.
FAU - Moriki, T
AU  - Moriki T
AD  - Department of Pathology, Kochi Medical School, Japan.
FAU - Kimura, S
AU  - Kimura S
FAU - Yamane, T
AU  - Yamane T
FAU - Hara, H
AU  - Hara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Acta Pathol Jpn
JT  - Acta pathologica japonica
JID - 0372637
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
RN  - 10028-17-8 (Tritium)
RN  - F30N4O6XVV (Acridine Orange)
RN  - VC2W18DGKR (Thymidine)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - Acridine Orange/*analysis/metabolism
MH  - Animals
MH  - Cell Line, Transformed/analysis/ultrastructure
MH  - Cell Nucleus/analysis
MH  - Chromatin/*analysis/metabolism
MH  - Cricetinae
MH  - DNA, Neoplasm/metabolism
MH  - DNA, Viral/metabolism
MH  - Fibrosarcoma/pathology
MH  - Microscopy, Electron
MH  - Simplexvirus/*physiology
MH  - Thymidine/metabolism
MH  - Tritium
MH  - Tumor Cells, Cultured
MH  - Uridine/metabolism
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1988.tb02341.x [doi]
PST - ppublish
SO  - Acta Pathol Jpn. 1988 Jun;38(6):693-704. doi: 10.1111/j.1440-1827.1988.tb02341.x.