PMID- 28512854
OWN - NLM
STAT- MEDLINE
DCOM- 20171004
LR  - 20181202
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 31
IP  - 7
DP  - 2017 Jul
TI  - Mogroside IIIE Attenuates LPS-Induced Acute Lung Injury in Mice Partly Through 
      Regulation of the TLR4/MAPK/NF-kappaB Axis via AMPK Activation.
PG  - 1097-1106
LID - 10.1002/ptr.5833 [doi]
AB  - Acute lung injury (ALI) often leads to high mortality, and there is as yet no 
      effective drug treatment. The present study aimed to investigate protective 
      effects of mogroside IIIE (MGIIIE, a cucurbitane-type triterpenoid from Siraitia 
      grosvenorii Fruits) in experimental ALI and its underlying mechanism. MGIIIE (1, 
      10 0r 20 mg/kg) was orally administered for 1 h before a single intratracheal 
      administration of lipopolysaccharide (LPS, 5 mg/kg). MGIIIE treatment 
      dose-dependently suppressed pulmonary oedema, pro-inflammatory mediators (IL-1beta, 
      IL-6, TNF-alpha and HMGB1) release and higher MPO activity in lung tissues induced by 
      LPS challenge. Molecular researches showed that mogroside IIIE (20 mg/kg) not 
      only increased the phosphorylation of adenosine 5'-monophosphate-activated 
      protein kinase (AMPK) but suppressed the over-expression of toll-like receptor 4 
      (TLR4) and myeloid differentiation factor 88 (MyD88). In addition, MGIIIE also 
      inhibited the activation of MAPKs and nuclear factor kappaB (NF-kappaB) signalling in 
      lung tissues from LPS-challenged mice. Similar antiinflammatory effects of MGIIIE 
      were obtained in LPS-treated macrophages. Compound C (a pharmacological AMPK 
      inhibitor) obviously reversed the antiinflammatory effect of MGIIIE in 
      LPS-induced ALI mice. Taken together, AMPK activation plays a crucial role in the 
      antiinflammatory effects of MGIIIE in LPS-induced ALI by down-regulating 
      TLR4/MAPK/NF-kappaB signalling pathways. Copyright (c) 2017 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Tao, Lijun
AU  - Tao L
AD  - Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 
      211198, People's Republic of China.
FAU - Cao, Fengyan
AU  - Cao F
AD  - Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 
      211198, People's Republic of China.
FAU - Xu, Gonghao
AU  - Xu G
AD  - Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 
      211198, People's Republic of China.
FAU - Xie, Haifeng
AU  - Xie H
AD  - Chengdu Biopurity Chengdu Biopurity Phytochemicals Ltd, Chengdu, 611131, People's 
      Republic of China.
FAU - Zhang, Mian
AU  - Zhang M
AUID- ORCID: 0000-0002-1504-305X
AD  - Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 
      211198, People's Republic of China.
FAU - Zhang, Chaofeng
AU  - Zhang C
AD  - Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 
      211198, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20170517
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Glucosides)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, mouse)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Triterpenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - 0 (mogroside IIIE)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Acute Lung Injury/chemically induced/*drug therapy
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Down-Regulation
MH  - Glucosides/*pharmacology
MH  - HMGB1 Protein
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides
MH  - Lung/drug effects
MH  - Macrophages/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - NF-kappa B/*metabolism
MH  - Phosphorylation
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/*metabolism
MH  - Triterpenes/*pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - AMPK
OT  - TLR4
OT  - acute lung injury
OT  - lipopolysaccharide
OT  - mogroside IIIE
EDAT- 2017/05/18 06:00
MHDA- 2017/10/05 06:00
CRDT- 2017/05/18 06:00
PHST- 2017/01/22 00:00 [received]
PHST- 2017/03/24 00:00 [revised]
PHST- 2017/04/19 00:00 [accepted]
PHST- 2017/05/18 06:00 [pubmed]
PHST- 2017/10/05 06:00 [medline]
PHST- 2017/05/18 06:00 [entrez]
AID - 10.1002/ptr.5833 [doi]
PST - ppublish
SO  - Phytother Res. 2017 Jul;31(7):1097-1106. doi: 10.1002/ptr.5833. Epub 2017 May 17.