PMID- 28514158 OWN - NLM STAT- MEDLINE DCOM- 20190123 LR - 20190123 IS - 1520-5827 (Electronic) IS - 0743-7463 (Linking) VI - 33 IP - 23 DP - 2017 Jun 13 TI - Preparation of Carbon Dots for Cellular Imaging by the Molecular Aggregation of Cellulolytic Enzyme Lignin. PG - 5786-5795 LID - 10.1021/acs.langmuir.7b00617 [doi] AB - Carbon dots, which are less than 10 nm in diameter, have been widely investigated because of their unique luminescence properties and potential for use in bioimaging. In the present work, natural carbon dots (L-CDs) were obtained by molecular aggregation, using ethanol-extracted cellulolytic enzyme lignin. The whole process for the preparation of L-CDs was green and simple to operate and did not use toxic chemical reagents or harsh conditions. The newly prepared L-CDs emitted multicolor photoluminescence following one- and two-photon excitation. The L-CDs also showed good cellular biocompatibility, which is crucial for biological applications. One- and two-photon cell-imaging studies demonstrated the potential of L-CDs for bioimaging. FAU - Niu, Na AU - Niu N FAU - Ma, Zhuoming AU - Ma Z FAU - He, Fei AU - He F AD - Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University , Harbin 150001, P.R. China. FAU - Li, Shujun AU - Li S AUID- ORCID: 0000-0002-3836-5350 FAU - Li, Jian AU - Li J FAU - Liu, Shouxin AU - Liu S FAU - Yang, Piaoping AU - Yang P AUID- ORCID: 0000-0002-9555-1803 AD - Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University , Harbin 150001, P.R. China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170530 PL - United States TA - Langmuir JT - Langmuir : the ACS journal of surfaces and colloids JID - 9882736 RN - 7440-44-0 (Carbon) RN - 9004-34-6 (Cellulose) RN - 9005-53-2 (Lignin) SB - IM MH - Carbon/*chemistry MH - Cell Line, Tumor MH - Cellulose MH - Humans MH - Lignin MH - Luminescence MH - Molecular Imaging MH - Quantum Dots EDAT- 2017/05/18 06:00 MHDA- 2019/01/24 06:00 CRDT- 2017/05/18 06:00 PHST- 2017/05/18 06:00 [pubmed] PHST- 2019/01/24 06:00 [medline] PHST- 2017/05/18 06:00 [entrez] AID - 10.1021/acs.langmuir.7b00617 [doi] PST - ppublish SO - Langmuir. 2017 Jun 13;33(23):5786-5795. doi: 10.1021/acs.langmuir.7b00617. Epub 2017 May 30.