PMID- 28515079
OWN - NLM
STAT- MEDLINE
DCOM- 20170816
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 313
IP  - 1
DP  - 2017 Jul 1
TI  - Impact of acetaminophen consumption and resistance exercise on extracellular 
      matrix gene expression in human skeletal muscle.
PG  - R44-R50
LID - 10.1152/ajpregu.00019.2017 [doi]
AB  - Acetaminophen (APAP) given during chronic exercise reduces skeletal muscle 
      collagen and cross-linking in rats. We propose that the effect of APAP on muscle 
      extracellular matrix (ECM) may, in part, be mediated by dysregulation of the 
      balance between matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs 
      (TIMPs). The purpose of this study was to evaluate the impact of APAP consumption 
      during acute resistance exercise (RE) on several regulators of the ECM in human 
      skeletal muscle. In a double-blinded, placebo-controlled, randomized crossover 
      design, recreationally active men (n = 8, 25 +/- 2 yr) performed two trials of knee 
      extension. Placebo (PLA) or APAP (1,000 mg/6 h) was given for 24 h before and 
      immediately following RE. Vastus lateralis biopsies were taken at baseline and 1 
      and 3 h post-RE. Quantitative RT-PCR was used to determine differences in mRNA 
      expression. MMP-2, type I collagen, and type III collagen mRNA expression was not 
      altered by exercise or APAP (P > 0.05). When compared with PLA, TIMP-1 expression 
      was lower at 1 h post-RE during APAP conditions but greater than PLA at 3 h 
      post-RE (P < 0.05). MMP-9 expression and protein levels were elevated at 3 h 
      post-RE independent of treatment (P < 0.05). Lysyl oxidase expression was greater 
      at 3 h post-RE during APAP consumption (P < 0.05) compared with PLA. MMP-2 and 
      TIMP-1 protein was not altered by RE or APAP (P > 0.05). Phosphorylation of 
      ERK1/2 and p38-MAPK increased (P < 0.05) with RE but was not influenced by APAP. 
      Our findings do not support our hypothesis and suggest that short-term APAP 
      consumption before RE has a small impact on the measured ECM molecules in human 
      skeletal muscle following acute RE.
CI  - Copyright (c) 2017 the American Physiological Society.
FAU - Patel, Shivam H
AU  - Patel SH
AD  - Department of Physiology, Midwestern University, Glendale, Arizona.
AD  - Department of Health and Kinesiology, Purdue University, West Lafayette, Indiana.
FAU - D'Lugos, Andrew C
AU  - D'Lugos AC
AD  - School of Nutrition and Health Promotion, Healthy Lifestyles Research Center, 
      Exercise Science and Health Promotion, Arizona State University, Phoenix, 
      Arizona; and.
FAU - Eldon, Erica R
AU  - Eldon ER
AD  - Department of Physiology, Midwestern University, Glendale, Arizona.
FAU - Curtis, Donald
AU  - Curtis D
AD  - Podiatry, Midwestern University, Glendale, Arizona.
FAU - Dickinson, Jared M
AU  - Dickinson JM
AD  - School of Nutrition and Health Promotion, Healthy Lifestyles Research Center, 
      Exercise Science and Health Promotion, Arizona State University, Phoenix, 
      Arizona; and.
FAU - Carroll, Chad C
AU  - Carroll CC
AD  - Department of Physiology, Midwestern University, Glendale, Arizona; 
      carrol71@purdue.edu.
AD  - Department of Health and Kinesiology, Purdue University, West Lafayette, Indiana.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170517
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (RNA, Messenger)
RN  - 362O9ITL9D (Acetaminophen)
SB  - IM
MH  - Acetaminophen/*pharmacology
MH  - Adult
MH  - Exercise/*physiology
MH  - Extracellular Matrix/*drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Male
MH  - Muscle, Skeletal/*drug effects/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - exercise
OT  - extracellular matrix
OT  - paracetamol
EDAT- 2017/05/19 06:00
MHDA- 2017/08/17 06:00
CRDT- 2017/05/19 06:00
PHST- 2017/01/23 00:00 [received]
PHST- 2017/04/17 00:00 [revised]
PHST- 2017/05/05 00:00 [accepted]
PHST- 2017/05/19 06:00 [pubmed]
PHST- 2017/08/17 06:00 [medline]
PHST- 2017/05/19 06:00 [entrez]
AID - ajpregu.00019.2017 [pii]
AID - 10.1152/ajpregu.00019.2017 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2017 Jul 1;313(1):R44-R50. doi: 
      10.1152/ajpregu.00019.2017. Epub 2017 May 17.