PMID- 2851744
OWN - NLM
STAT- MEDLINE
DCOM- 19890323
LR  - 20031114
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 35
IP  - 6
DP  - 1988
TI  - Concentration of retroviruses by low-speed centrifugation.
PG  - 651-5
AB  - Concentration of retroviruses from volumes of up to 6 liters of medium by 
      low-speed centrifugation is described. In contrast to pelleting, no damage or 
      aggregation of particles was observed. Surface glycoproteins were also fully 
      preserved. This method enables simple handling of relatively large volumes of 
      medium. Highly purified mouse mammary tumor virus (MMTV) was obtained and its 
      transframe protein p30 in SDS-PAGE was recognized as a double band.
FAU - Prachar, J
AU  - Prachar J
AD  - Cancer Research Institute, Slovak Academy of Sciences, Bratislava, 
      Czechoslovakia.
FAU - Hlubinova, K
AU  - Hlubinova K
FAU - Kovarik, A
AU  - Kovarik A
FAU - Feldsamova, A
AU  - Feldsamova A
FAU - Simkovic, D
AU  - Simkovic D
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Centrifugation/methods
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Mammary Tumor Virus, Mouse/*isolation & purification
MH  - Molecular Weight
MH  - RNA, Viral/biosynthesis/isolation & purification
MH  - Retroviridae/*isolation & purification
MH  - Viral Proteins/isolation & purification
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Neoplasma. 1988;35(6):651-5.