PMID- 28524080 OWN - NLM STAT- MEDLINE DCOM- 20180314 LR - 20181113 IS - 1660-3397 (Electronic) IS - 1660-3397 (Linking) VI - 15 IP - 5 DP - 2017 May 18 TI - Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells. LID - 10.3390/md15050140 [doi] LID - 140 AB - In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 degrees C; and an extraction time of 3 h. Then, a purified polysaccharide named Cereus sinensis polysaccharide-1 (CSP-1) was obtained from the crude polysaccharide by the Diethylaminoethyl cellulose-52 (DEAE-52) cellulose chromatography column and Sephadex G-100 column. The molecular weight and monosaccharide composition of CSP-1 was determined through Gel Permeation Chromatography (GPC) and Gas Chromatography-Mass Spectrometer (GS-MS), respectively. The results showed that CSP-1 with an average molecular weight of 56,335 Da was composed of l-(-)-Fucose, d-(+)-Mannose, d-Glucose and mainly possessed 1-->2, 1-->2, 6, 1-->4, and 1-->4, 6 of glycosyl linkages. The immunomodulatory activities of CSP-1 were also evaluated using lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. The results demonstrated that CSP-1 dose-dependently protected against LPS-induced toxicity, and CSP-1 significantly inhibited the Toll-like receptor 4 (TLR-4) mRNA, myeloid differentiation factor 88 (MyD88) mRNA and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA expression of the LPS-induced THP-1 cells, as well as suppressing reactive oxygen species (ROS) generation. FAU - Wu, Junwen AU - Wu J AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. 15800711801@163.com. FAU - Zhou, Xuefei AU - Zhou X AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. zhouxuefeibest@163.com. FAU - Zhang, Min AU - Zhang M AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. 13122223561@163.com. FAU - Yao, Yun AU - Yao Y AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. m150208413@st.shou.edu.cn. FAU - Han, Juanjuan AU - Han J AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. jjhan1409@163.com. FAU - Liu, Kehai AU - Liu K AD - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. khliu@shou.edu.cn. LA - eng PT - Journal Article DEP - 20170518 PL - Switzerland TA - Mar Drugs JT - Marine drugs JID - 101213729 RN - 0 (Polysaccharides) SB - IM MH - Animals MH - Cell Survival/drug effects MH - Humans MH - Monocytes/*drug effects MH - Polysaccharides/chemistry/*pharmacology MH - Sea Anemones/*chemistry MH - THP-1 Cells/drug effects PMC - PMC5450546 OTO - NOTNLM OT - Cereus sinensis OT - THP-1 cells OT - immunomodulatory property OT - polysaccharide COIS- The authors declare no conflict of interest. EDAT- 2017/05/20 06:00 MHDA- 2018/03/15 06:00 PMCR- 2017/05/01 CRDT- 2017/05/20 06:00 PHST- 2017/03/16 00:00 [received] PHST- 2017/04/24 00:00 [revised] PHST- 2017/05/04 00:00 [accepted] PHST- 2017/05/20 06:00 [entrez] PHST- 2017/05/20 06:00 [pubmed] PHST- 2018/03/15 06:00 [medline] PHST- 2017/05/01 00:00 [pmc-release] AID - md15050140 [pii] AID - marinedrugs-15-00140 [pii] AID - 10.3390/md15050140 [doi] PST - epublish SO - Mar Drugs. 2017 May 18;15(5):140. doi: 10.3390/md15050140.